Analytical Data
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基因名
DDX53
- Application
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别名
DDX53；CAGE；Probable ATP-dependent RNA helicase DDX53
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q86TM3
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表达区间
1-631aa
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氨基酸序列
MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS
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分子量
76.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DDX53, also known as DEAD-box helicase 53, is a member of the DEAD-box protein family, which is characterized by the presence of a conserved helicase core that plays critical roles in RNA metabolism, including RNA unwinding, splicing, and ribosome biogenesis. This protein has garnered interest due to its potential involvement in various cellular processes and diseases, particularly cancer. Recent studies have indicated that DDX53 may contribute to the regulation of gene expression and RNA processing, influencing pathways that are crucial for cell proliferation and survival. Moreover, aberrant expression of DDX53 has been implicated in tumorigenesis, making it a target of investigation for therapeutic interventions. The recombinant expression of DDX53 allows for a detailed functional analysis of this protein in vitro, facilitating studies on its interactions with other molecular partners and its role in relevant biological processes. Through the production of DDX53 as a recombinant protein, researchers aim to elucidate its structure-function relationship, investigate its potential as a biomarker, and explore its applicability in developing new treatment strategies for cancers and other diseases associated with disrupted RNA metabolism. The insights gained from such studies could pave the way for targeted therapies and enhance our understanding of the molecular underpinnings of diseases linked to DDX53 dysregulation.
