Analytical Data
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Gene name
DDX53
- Application
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Alternative Names
DDX53；CAGE；Probable ATP-dependent RNA helicase DDX53
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86TM3
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Expression Region
1-631aa
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AA Sequence
MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS
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Molecular Weight
76.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DDX53, also known as DEAD-box helicase 53, is a member of the DEAD-box protein family, which is characterized by the presence of a conserved helicase core that plays critical roles in RNA metabolism, including RNA unwinding, splicing, and ribosome biogenesis. This protein has garnered interest due to its potential involvement in various cellular processes and diseases, particularly cancer. Recent studies have indicated that DDX53 may contribute to the regulation of gene expression and RNA processing, influencing pathways that are crucial for cell proliferation and survival. Moreover, aberrant expression of DDX53 has been implicated in tumorigenesis, making it a target of investigation for therapeutic interventions. The recombinant expression of DDX53 allows for a detailed functional analysis of this protein in vitro, facilitating studies on its interactions with other molecular partners and its role in relevant biological processes. Through the production of DDX53 as a recombinant protein, researchers aim to elucidate its structure-function relationship, investigate its potential as a biomarker, and explore its applicability in developing new treatment strategies for cancers and other diseases associated with disrupted RNA metabolism. The insights gained from such studies could pave the way for targeted therapies and enhance our understanding of the molecular underpinnings of diseases linked to DDX53 dysregulation.
