Analytical Data
-
Gene name
CPNE4
- Application
-
Alternative Names
CPNE4Copine-4; Copine IV; Copine-8
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96A23
-
Expression Region
1-557aa
-
AA Sequence
MKKMSNIYESAANTLGIFNSPCLTKVELRVACKGISDRDALSKPDPCVILKMQSHGQWFEVDRTEVIRTCINPVYSKLFTVDFYFEEVQRLRFEVHDISSNHNGLKEADFLGGMECTLGQIVSQRKLSKSLLKHGNTAGKSSITVIAEELSGNDDYVELAFNARKLDDKDFFSKSDPFLEIFRMNDDATQQLVHRTEVVMNNLSPAWKSFKVSVNSLCSGDPDRRLKCIVWDWDSNGKHDFIGEFTSTFKEMRGAMEGKQVQWECINPKYKAKKKNYKNSGTVILNLCKIHKMHSFLDYIMGGCQIQFTVAIDFTASNGDPRNSCSLHYIHPYQPNEYLKALVAVGEICQDYDSDKMFPAFGFGARIPPEYTVSHDFAINFNEDNPECAGIQGVVEAYQSCLPKLQLYGPTNIAPIIQKVAKSASEETNTKEASQYFILLILTDGVITDMADTREAIVHASHLPMSVIIVGVGNADFSDMQMLDGDDGILRSPKGEPVLRDIVQFVPFRNFKHASPAALAKSVLAEVPNQVVDYYNGKGIKPKCSSEMYESSRTLAP
-
Molecular Weight
88.8 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CPNE4 (Copine 4) is a member of the copine family of calcium-dependent membrane-binding proteins that play crucial roles in various cellular processes, including signal transduction, cell adhesion, and membrane trafficking. Research into CPNE4 has gained traction due to its potential implications in neurobiology and its involvement in neurodegenerative diseases. Studies have indicated that CPNE4 might regulate synaptic plasticity and neuronal survival, making it a target of interest for understanding mechanisms underlying cognitive functions and neurodegeneration. The ability of CPNE4 to interact with phospholipids in a calcium-dependent manner raises questions about its role in membrane dynamics and cellular signaling pathways. Moreover, the protein's expression patterns in the brain suggest that it could be instrumental in modulating synaptic strength and plasticity, which are critical for learning and memory. As such, the recombinant form of CPNE4 is being investigated to elucidate its functional properties and interactions within neuronal contexts. By producing CPNE4 as a recombinant protein, researchers aim to explore its binding affinities, structural characteristics, and potential therapeutic implications, contributing to a better understanding of its role in health and disease. Overall, the study of CPNE4 and its recombinant forms represents a significant step towards uncovering novel insights into calcium-dependent mechanisms within the nervous system and their relevance to neurodegenerative conditions.
