Analytical Data
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基因名
SLC12A8
- Application
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别名
SLC12A8; CCC9; Solute carrier family 12 member 8; Cation-chloride cotransporter 9
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A0AV02
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氨基酸序列
MTQMSQVQELFHEAAQQDALAQPQPWWKTQLFMWEPVLFGTWDGVFTSCMINIFGVVLFLRTGWLVGNTGVLLGMFLVSFVILVALVTVLSGIGVGERSSIGSGGVYSMISSVLGGQTGGTIGLLYVFGQCVAGAMYITGFAESISDLLGLGNIWAVRGISVAVLLALLGINLAGVKWIIRLQLLLLFLLAVSTLDFVVGSFTHLDPEHGFIGYSPELLQNNTLPDYSPGESFFTVFGVFFPAATGVMAGFNMGGDLREPAASIPLGSLAAVGISWFLYIVFVFLLGAICTREALRYDFLIAEKVSLMGFLFLLGLYISSLASCMGGLYGAPRILQCIAQEKVIPALACLGQGKGPNKTPVAAICLTSLVTMAFVFVGQVNVLAPIVTINFMLTYVAVDYSYFSLSMCSCSLTPVPEPVLREGAEGLHCSEHLLLEKAPSYGSEGPAQRVLEGTLLEFTKDMDQLLQLTRKLESSQPRQGEGNRTPESQKRKSKKATKQTLQDSFLLDLKSPPSFPVEISDRLPAASWEGQESCWNKQTSKSEGTQPEGTYGEQLVPELCNQSESSGEDFFLKSRLQEQDVWRRSTSFYTHMCNPWVSLLGAVGSLLIMFVIQWVYTLVNMGVAAIVYFYIGRASPGLHLGSASNFSFFRWMRSLLLPSCRSLQSPQEQIILAPSLAKVDMEMTQLTQENADFATRDRYHHSSLVNREQLMPHY
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分子量
78.59 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC12A8, a member of the SLC12 family of solute carriers, encodes a sodium-dependent cation chloride cotransporter primarily expressed in the kidney and other tissues. Its role in ion transport and homeostasis has garnered significant interest, especially in the context of renal physiology and potential implications in hypertension and electrolyte imbalances. Recent studies suggest that SLC12A8 may be involved in the regulation of sodium and chloride reabsorption, which are critical processes for maintaining fluid balance and blood pressure in the body. Additionally, aberrations in SLC12A8 expression or function have been linked to various pathological conditions, including renal disorders and cardiovascular diseases. The recombinant expression of SLC12A8 protein in heterologous systems is essential for elucidating its molecular mechanisms and functional characteristics. Understanding the structure-function relationship of SLC12A8 through protein engineering and biophysical studies can reveal potential therapeutic targets for diseases associated with ion transport dysregulation. Furthermore, recombinant SLC12A8 can serve as a valuable tool for drug screening and development, aimed at modulating its activity to restore proper ionic balance in diseased states. Thus, research on SLC12A8 recombinant protein is pivotal for advancing our knowledge of renal function and identifying novel interventions for managing related health conditions.
