Analytical Data
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Gene name
HIST3H2A
- Application
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Alternative Names
HIST3H2A；H2AW；HIST3H2A；Histone H2A type 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7L7L0
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Expression Region
2-130aa
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AA Sequence
SGRGKQGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGNY SERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGR VTIAQGGVLP NIQAVLLPKK TESHHKAKGK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HIST3H2A is a histone protein that plays a crucial role in the structure and function of chromatin, which is essential for regulating gene expression and maintaining genomic integrity. As a key component of the nucleosome, HIST3H2A participates in the packaging of DNA into a compact form, influencing various cellular processes such as DNA replication, repair, and transcription. Understanding the molecular mechanisms involving HIST3H2A is vital for deciphering the complex regulation of gene expression and the implications of histone modification in diseases, including cancer and genetic disorders. The study of recombinant HIST3H2A proteins allows researchers to investigate their biochemical properties, interactions with DNA and other proteins, and the effects of post-translational modifications. Moreover, recombinant histones can be used to create versatile in vitro models for exploring chromatin dynamics and the impact of histone variants on cellular functions. This research not only enhances our understanding of epigenetic regulation but also opens avenues for potential therapeutic strategies targeting chromatin-related pathways. Overall, the investigation of HIST3H2A and its recombinant forms is a critical area of research in molecular biology, with significant implications for genetics, epigenetics, and disease treatment.
