Analytical Data
-
Gene name
COX15
- Application
-
Alternative Names
CEMCOX2; COX15; COX15 homolog; cytochrome c oxidase assembly protein; COX15; S. cerevisiae; homolog of; COX15_HUMAN;
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q7KZN9
-
Expression Region
1-410aa
-
AA Sequence
MQRLLFPPLRALKGRQYLPLLAPRAAPRAQCDCIRRPLRPGQYSTISEVALQSGRGTVSLPSKAAERVVGRWLLVCSGTVAGAVILGGVTRLTESGLSMVDWHLIKEMKPPTSQEEWEAEFQRYQQFPEFKILNHDMTLTEFKFIWYMEYSHRMWGRLVGLVYILPAAYFWRKGWLSRGMKGRVLALCGLVCFQGLLGWYMVKSGLEEKSDSHDIPRVSQYRLAAHLGSALVLYCASLWTSLSLLLPPHKLPETHQLLQLRRFAHGTAGLVFLTALSGAFVAGLDAGLVYNSFPKMGESWIPEDLFTFSPILRNVFENPTMVQFDHRILGITSVTAITVLYFLSRRIPLPRRTKMAAVTLLALAYTQVGLGISTLLMYVPTPLAATHQSGSLALLTGALWLMNELRRVPK
-
Molecular Weight
70.73 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
COX15, a crucial gene involved in the biogenesis of cytochrome c oxidase (COX), plays an essential role in mitochondrial function and cellular respiration. This gene encodes a protein that is integral to the assembly and maturation of the COX complex, which is the terminal enzyme of the mitochondrial electron transport chain (ETC). Deficiencies or mutations in COX15 have been associated with various mitochondrial diseases, leading to impaired oxidative phosphorylation and resulting in a range of metabolic disorders. Research on COX15 recombinant protein aims to elucidate its structural and functional characteristics, enabling a better understanding of its role in the assembly of mitochondrial respiratory complexes. Studies have shown that COX15 is involved in the synthesis of heme a, a critical cofactor for COX activity. By generating and studying the recombinant COX15 protein, researchers seek to determine the enzymatic properties, interaction dynamics with other mitochondrial proteins, and the impact of specific mutations on its function. This understanding has significant implications for developing targeted therapies for mitochondrial diseases caused by COX dysfunction. Furthermore, insights gained from COX15 studies can contribute to broader mitochondrial research, offering potential pathways for therapeutic intervention and improved management of metabolic disorders associated with mitochondrial dysfunction.
