Analytical Data
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Gene name
SIX2
- Application
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Alternative Names
Homeobox protein SIX2; OTTHUMP00000201649; Sine oculis homeobox (Drosophila) homolog 2; Sine oculis homeobox homolog 2 (Drosophila); Sine oculis homeobox homolog 2; SIX homeobox 2; Six2; SIX2_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NPC8
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Expression Region
1-291 aa
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AA Sequence
MSMLPTFGFT QEQVACVCEV LQQGGNIERL GRFLWSLPAC EHLHKNESVL KAKAVVAFHR GNFRELYKIL ESHQFSPHNH AKLQQLWLKA HYIEAEKLRG RPLGAVGKYR VRRKFPLPRS IWDGEETSYC FKEKSRSVLR EWYAHNPYPS PREKRELAEA TGLTTTQVSN WFKNRRQRDR AAEAKERENN ENSNSNSHNP LNGSGKSVLG SSEDEKTPSG TPDHSSSSPA LLLSPPPPGL PSLHSLGHPP GPSAVPVPVP GGGGADPLQH HHGLQDSILN PMSANLVDLG S
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Molecular Weight
32.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SIX2 (Sine Oculis Homeobox 2) is a transcription factor known to play a crucial role in various biological processes, particularly in kidney development and organogenesis. Research on SIX2 has gained significant momentum due to its involvement in the regulation of nephron development, making it a key player in understanding congenital kidney diseases and potential regenerative therapies. Studies have shown that SIX2 is essential for maintaining the renal progenitor cell population during embryonic development and is linked to signaling pathways that influence cell fate decisions. Moreover, aberrations in SIX2 expression are associated with developmental disorders, such as congenital abnormalities of the kidney and urinary tract (CAKUT). Investigating the recombinant forms of SIX2 protein has enabled researchers to delineate its functional domains and regulatory mechanisms, further elucidating its role in gene expression and cellular differentiation. Through techniques like recombinant DNA technology, scientists can produce and manipulate SIX2 protein in vitro, aiding in the exploration of its molecular interactions and potential therapeutic applications. This ongoing research not only enhances our comprehension of renal biology but also holds promise for innovative strategies in treating kidney-related disorders and advancing regenerative medicine. Overall, the study of recombinant SIX2 protein serves as a vital link between basic science and clinical applications, highlighting its significance in developmental biology and translational research.
