Analytical Data
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Gene name
COQ3
- Application
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Alternative Names
COQ3; UG0215E05Ubiquinone biosynthesis O-methyltransferase; mitochondrial; 3-demethylubiquinol 3-O-methyltransferase; EC 2.1.1.64; Polyprenyldihydroxybenzoate methyltransferase; EC 2.1.1.114
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NZJ6
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Expression Region
1-369aa
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AA Sequence
MWSGRKLGSSGGWFLRVLGPGGCNTKAARPLISSAVYVKNQLSGTLQIKPGVFNEYRTIWFKSYRTIFSCLNRIKSFRYPWARLYSTSQTTVDSGEVKTFLALAHKWWDEQGVYAPLHSMNDLRVPFIRDNLLKTIPNHQPGKPLLGMKILDVGCGGGLLTEPLGRLGASVIGIDPVDENIKTAQCHKSFDPVLDKRIEYRVCSLEEIVEETAETFDAVVASEVVEHVIDLETFLQCCCQVLKPGGSLFITTINKTQLSYALGIVFSEQIAGIVPKGTHTWEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGETEELQANACTNPAVHEKLKK
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Molecular Weight
67.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COQ3, or Coenzyme Q3, is a crucial enzyme involved in the biosynthesis of ubiquinone (coenzyme Q), an essential lipid-soluble antioxidant that plays vital roles in cellular respiration and energy production within mitochondria. The importance of COQ3 in maintaining mitochondrial function and overall cellular health has sparked interest in its study, particularly in relation to various human diseases, including neurodegenerative disorders and metabolic syndromes. Deficiencies or mutations in the COQ3 gene can lead to impaired coenzyme Q biosynthesis, resulting in reduced energy production and increased oxidative stress, which are implicated in the pathogenesis of conditions like Parkinson's disease and other mitochondrial disorders. Investigating the recombinant expression of COQ3 protein can provide insights into its functional mechanisms, structural characteristics, and potential as a therapeutic target. By producing COQ3 in a controlled laboratory setting, researchers can study its enzymatic activity, interaction with other biomolecules, and the impact of various mutations. Furthermore, understanding the role of COQ3 can aid in developing strategies for enhancing coenzyme Q levels in therapeutic applications, ultimately contributing to the advancement of treatments for mitochondrial dysfunction and related diseases. Overall, the study of COQ3 recombinant protein offers a promising avenue for connecting basic biochemical research with potential clinical applications, addressing both fundamental questions in cellular metabolism and practical challenges in treating mitochondrial diseases.
