Analytical Data
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Gene name
GRK6
- Application
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Alternative Names
GRK6；GPRK6；G Protein-coupled receptor kinase 6
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43250
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Expression Region
1-576aa
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AA Sequence
MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ PIGRLLFREF CATRPELSRC VAFLDGVAEY EVTPDDKRKA CGRQLTQNFL SHTGPDLIPE VPRQLVTNCT QRLEQGPCKD LFQELTRLTH EYLSVAPFAD YLDSIYFNRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVPEEYSE RFSPQARSLC SQLLCKDPAE RLGCRGGSAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI EQFSTVKGVE LEPTDQDFYQ KFATGSVPIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK GQPPAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GRK6, or G protein-coupled receptor kinase 6, is a critical enzyme involved in the regulation of G protein-coupled receptors (GPCRs), which are pivotal for transducing extracellular signals into cellular responses. The study of GRK6 has gained prominence due to its essential role in mediating various physiological processes and its implications in pathological conditions, including heart failure and neurodegenerative diseases. Recent research has highlighted the significance of GRK6 in receptor desensitization, phosphorylation, and the overall modulation of signaling pathways that govern cell function. Moreover, aberrations in GRK6 activity can influence the pharmacological responses of many therapeutic agents, making it an attractive target for drug development. Investigating the recombinant expression and characterization of GRK6 can provide insights into its enzymatic activity, substrate specificity, and interactions with other proteins. Such studies are crucial for understanding the molecular mechanisms underpinning GRK6's function in health and disease, and they may pave the way for the development of novel therapeutics aimed at modulating its activity for clinical benefit. The evolution of recombinant protein technology has enabled researchers to produce GRK6 in a controlled environment, facilitating detailed structural and functional studies that were previously challenging, thus expanding our understanding of GPCR signaling dynamics and potentially leading to innovative treatment strategies.
