Analytical Data
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Gene name
ATG14
- Application
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Alternative Names
ATG14；ATG14L；KIAA0831；Beclin 1-associated autophagy-related key regulator
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ZNE5
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Expression Region
1-492aa
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AA Sequence
MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR
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Molecular Weight
60.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ATG14 is a crucial protein that plays a significant role in the regulation of autophagy, a cellular process that maintains homeostasis by degrading and recycling cellular components. Autophagy is vital for cellular health, as it helps in eliminating damaged organelles and proteins, thereby preventing various diseases, including cancer and neurodegenerative disorders. ATG14 specifically is an essential component of the pre-autophagosomal structure (PAS) and functions in the recruitment of other proteins necessary for autophagosome formation. Research into ATG14's structure and function has revealed its involvement in the phosphoinositide signaling pathways that govern vesicle formation and trafficking within the cell. Moreover, studies have indicated that alterations in ATG14 expression or function can lead to impaired autophagy and contribute to disease pathology, making it a potential therapeutic target. Understanding ATG14's mechanisms and interactions with other autophagy-related proteins can provide insights into the intricate regulatory networks of autophagy, paving the way for novel strategies in disease treatment and management. Researchers are increasingly focusing on ATG14 through various biochemical and genetic approaches to unravel its full spectrum of functions, collaborations, and implications in cellular health and disease states.
