Analytical Data
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Gene name
CHEK2
- Application
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Alternative Names
CHEK2；CDS1；CHK2；RAD53；Serine/threonine-Protein kinase Chk2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O96017
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Expression Region
1-543aa
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AA Sequence
MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS QSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQ DGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREV GPKNSYIAYIEDHSGNGTFVNTELVGKGKR RPLNNNSEIALSLSRNKV FVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAI KIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYY IVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDL KPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVS VGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFI PEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDL LSEENESTALPQVLAQPSTSRKRPREGEAEGAETTKRPAVCAAVL
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Molecular Weight
88 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHEK2, or checkpoint kinase 2, is a crucial protein involved in the DNA damage response and cell cycle regulation, playing a significant role in maintaining genomic stability. Mutations in the CHEK2 gene are associated with an increased risk of various cancers, particularly breast and prostate cancers. The understanding of CHEK2 function and its implications in cancer biology has prompted extensive research into its structure and mechanism. Recombinant CHEK2 protein production enables scientists to investigate its biochemical properties, interaction with other cellular proteins, and its role in signaling pathways that respond to DNA damage. By studying CHEK2 in a controlled environment, researchers aim to elucidate its functional dynamics, the consequences of its mutations, and potential therapeutic interventions. This research is vital for developing targeted cancer therapies that could exploit weaknesses in the tumor cells' DNA repair mechanisms, thereby improving treatment outcomes for patients with CHEK2-associated malignancies. Understanding the intricate workings of CHEK2 not only sheds light on fundamental cellular processes but also opens avenues for advancements in precision medicine.
