Analytical Data
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Gene name
SH2D1B
- Application
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Alternative Names
SH2D1B; EAT2; SH2 domain-containing protein 1B; EWS/FLI1-activated transcript 2; EAT-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14796
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Expression Region
1-132 aa
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AA Sequence
MDLPYYHGRL TKQDCETLLL KEGVDGNFLL RDSESIPGVL CLCVSFKNIV YTYRIFREKH GYYRIQTAEG SPKQVFPSLK ELISKFEKPN QGMVVHLLKP IKRTSPSLRW RGLKLELETF VNSNSDYVDV LP
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Molecular Weight
15.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SH2D1B, also known as Src Homology 2 Domain Containing 1B, is a protein that plays a crucial role in immune signaling pathways, particularly in T-cell activation and function. It interacts with various signaling molecules, influencing cellular responses to stimuli. The study of SH2D1B is particularly relevant in the context of autoimmune diseases and immunological disorders, where dysregulation of T-cell signaling can lead to pathological conditions. Research has shown that SH2D1B is involved in the modulation of pathways such as the JAK-STAT signaling cascade, which is vital for the development and differentiation of immune cells. Furthermore, SH2D1B is linked to the genetic basis of certain immunodeficiencies, where mutations can impair its function, highlighting its importance in maintaining immune homeostasis. The production of recombinant SH2D1B is essential for detailed biochemical and biophysical characterization, allowing researchers to better understand its structure-function relationship. Such studies can pave the way for the development of therapeutic strategies aimed at correcting the signaling defects associated with SH2D1B dysfunction. Overall, the exploration of SH2D1B as a recombinant protein provides valuable insights into the fundamental mechanisms of immune regulation, with implications for therapeutic interventions in immune-related diseases.
