Analytical Data
-
基因名
ligA
- Application
-
别名
ligA；HCA56；LGTN；Eukaryotic translation initiation factor 2D
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P15042
-
表达区间
1-671aa
-
氨基酸序列
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS
-
分子量
77.6 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Ligation of DNA is a fundamental process in molecular biology, and the enzyme DNA ligase plays a crucial role in this mechanism by facilitating the joining of DNA strands. Among the various types of DNA ligases, ligA is of particular interest due to its unique properties and potential applications in biotechnology and synthetic biology. This enzyme is primarily found in bacteria and is responsible for sealing nicks in the DNA backbone, thereby ensuring the integrity of genetic material during replication and repair. Research on ligA recombinant proteins has gained traction because of their utility in genetic engineering, where they can be employed to construct plasmids, modify genomic sequences, and facilitate the assembly of complex DNA constructs. Additionally, ligA’s ability to act on disparate DNA ends makes it a valuable tool for various applications, including gene therapy and the development of novel therapeutics. Understanding the structure and function of ligA, along with optimizing its activity through protein engineering, can lead to enhanced performance in laboratory settings. The study of ligA recombinant proteins not only contributes to the fundamental understanding of DNA ligation mechanisms but also holds promise for advancing techniques in molecular cloning and the development of DNA-based technologies, which are pivotal in fields ranging from medicine to agriculture. As researchers continue to explore the biochemical properties of ligA, there is significant potential for innovative applications that could arise from its use in synthetic biology and molecular diagnostics.
