Analytical Data
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Gene name
TLK1
- Application
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Alternative Names
TLK1；KIAA0137；Serine/threonine-Protein kinase tousled-like 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKI8
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Expression Region
1-766aa
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AA Sequence
MSVQSSSGSL EGPPSWSQLS TSPTPGSAAA ARSLLNHTPP SGRPREGAMD ELHSLDPRRQ ELLEARFTGV ASGSTGSTGS CSVGAKASTN NESSNHSFGS LGSLSDKESE TPEKKQSESS RGRKRKAENQ NESSQGKSIG GRGHKISDYF EYQGGNGSSP VRGIPPAIRS PQNSHSHSTP SSSVRPNSPS PTALAFGDHP IVQPKQLSFK IIQTDLTMLK LAALESNKIQ DLEKKEGRID DLLRANCDLR RQIDEQQKLL EKYKERLNKC ISMSKKLLIE KSTQEKLSSR EKSMQDRLRL GHFTTVRHGA SFTEQWTDGF AFQNLVKQQE WVNQQREDIE RQRKLLAKRK PPTANNSQAP STNSEPKQRK NKAVNGAEND PFVRPNLPQL LTLAEYHEQE EIFKLRLGHL KKEEAEIQAE LERLERVRNL HIRELKRINN EDNSQFKDHP TLNERYLLLH LLGRGGFSEV YKAFDLYEQR YAAVKIHQLN KSWRDEKKEN YHKHACREYR IHKELDHPRI VKLYDYFSLD TDTFCTVLEY CEGNDLDFYL KQHKLMSEKE ARSIVMQIVN ALRYLNEIKP PIIHYDLKPG NILLVDGTAC GEIKITDFGL SKIMDDDSYG VDGMDLTSQG AGTYWYLPPE CFVVGKEPPK ISNKVDVWSV GVIFFQCLYG RKPFGHNQSQ QDILQENTIL KATEVQFPVK PVVSSEAKAF IRRCLAYRKE DRFDVHQLAN DPYLLPHMRR SNSSGNLHMA GLTASPTPPS SSIITY
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Molecular Weight
135 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TLK1 (topoisomerase I-binding protein 1) is a crucial protein involved in DNA damage response and cell cycle regulation. Its role in facilitating the binding of topoisomerase I to DNA is pivotal for the maintenance of genomic integrity during DNA replication and repair. Research has indicated that TLK1 is essential for the proper functioning of various cellular processes, including chromatin remodeling and the regulation of protein kinases. Aberrant expression of TLK1 has been associated with several types of cancer, making it a potential target for therapeutic interventions. The recombinant expression of TLK1 allows researchers to obtain the protein in a purified form, enabling detailed biochemical and structural studies. These studies can help elucidate the molecular mechanisms by which TLK1 influences cell cycle dynamics and DNA repair pathways. Furthermore, understanding TLK1's interactions with key cellular partners may provide insights into its role in oncogenesis and tumor progression. This knowledge could contribute to the development of novel cancer treatments that harness TLK1's biological functions, providing a pathway for targeted therapies aimed at cancers characterized by TLK1 dysregulation. Consequently, the study of TLK1 recombinant protein is not only instrumental in basic research but also holds promise for advancing clinical applications in oncology.
