Analytical Data
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Gene name
CNOT4
- Application
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Alternative Names
CCR4 NOT transcription complex subunit 4; CCR4-associated factor 4; CCR4-NOT transcription complex subunit 4; CLONE243; CNOT 4; Cnot4; CNOT4_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95628
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Expression Region
1-565aa
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AA Sequence
MQCPKPDCMYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQRYDTPIDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVADNSSSIESLNMKEWQDGLRALLPNININFGGLPNSSSPSNANHSAPTSNTATTDSLSWDSPGSWTDPAIITGIPASSGNSLDSLQDDNPPHWLKSLQALTEMDGPSAAPSQTHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPQIYYRVQHWTAIRQRGATIRKCRICPTLFSPSQPTTHSSLLISYVLKNPVPDQFFFSLTPDAMRSQGHYHLFINCKNFC
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Molecular Weight
88.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CNOT4 is a critical component of the CCR4-NOT complex, which plays a vital role in various cellular processes, including mRNA deadenylation, degradation, and regulation of gene expression. As a member of the E3 ubiquitin ligase family, CNOT4 is instrumental in tagging substrates for proteasomal degradation, thereby influencing protein turnover and maintaining cellular homeostasis. Recent studies have indicated that CNOT4 is implicated in several biological processes, such as cell cycle regulation, stress responses, and developmental pathways. Dysregulation of CNOT4 has been associated with various diseases, including cancer, highlighting its potential as a therapeutic target. Understanding the structure and function of recombinant CNOT4 protein can provide insights into its mechanistic roles in cellular signaling pathways and its potential interactions with other molecular partners. This research is essential for elucidating the functional significance of CNOT4 and could lead to novel interventions in diseases linked to its dysregulation. Experimental approaches, including biochemical assays and structural biology techniques, are employed to characterize CNOT4 and investigate its ubiquitination activity and interactions. This accumulated knowledge can pave the way for developing strategies aimed at modulating CNOT4 activity for therapeutic benefits, making it a focal point in molecular biology and drug discovery.
