Analytical Data
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Gene name
choA
- Application
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Alternative Names
choA；Cholesterol oxidase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P12676
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Expression Region
43-546aa
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AA Sequence
DNGGYVPAVVIGTGYGAAVSALRLGEAGVQTLMLEMGQLWNQPGPDGNIFCGMLNPDKRSSWFKNRTEAPLGSFLWLDVVNRNIDPYAGVLDRVNYDQMSVYVGRGVGGGSLVNGGMAVEPKRSYFEEILPRVDSSEMYDRYFPRANSMLRVNHIDTKWFEDTEWYKFARVSREQAGKAGLGTVFVPNVYDFGYMQREAAGEVPKSALATEVIYGNNHGKQSLDKTYLAAALGTGKVTIQTLHQVKTIRQTKDGGYALTVEQKDTDGKLLATKEISCRYLFLGAGSLGSTELLVRARDTGTLPNLNSEVGAGWGPNGNIMTARANHMWNPTGAHQSSIPALGIDAWDNSDSSVFAEIAPMPAGLETWVSLYLAITKNPQRGTFVYDAATDRAKLNWTRDQNAPAVNAAKALFDRINKANGTIYRYDLFGTQLKAFADDFCYHPLGGCVLGKATDDYGRVAGYKNLYVTDGSLIPGSVGVNPFVTITALAERNVERIIKQDVTAS
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Molecular Weight
81.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ChoA, a choline O-acetyltransferase, plays a crucial role in the biosynthesis of the neurotransmitter acetylcholine, which is essential for nerve signal transmission in both peripheral and central nervous systems. The study of ChoA recombinant protein is significant due to its implications in understanding cholinergic signaling pathways, potential therapeutic targets for neurological disorders, and the overall mechanism of neurotransmitter synthesis. Research indicates that disruptions in acetylcholine production can lead to conditions such as Alzheimer's disease and myasthenia gravis. By investigating the structure and function of ChoA, scientists aim to elucidate its enzymatic properties and regulatory mechanisms, which may pave the way for the development of novel drugs that can modulate cholinergic activity. Furthermore, recombinant technology allows for the production of this protein in a controlled environment, enabling detailed studies of its kinetics, interactions with substrates, and influence of post-translational modifications. The insights gained from ChoA research may not only contribute to our understanding of neuronal function but also offer pathways to innovate treatments for acetylcholine-related disorders, highlighting the importance of this enzyme in both basic and applied biomedical research.
