Analytical Data
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Gene name
dnapkcs
- Application
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Alternative Names
dnapkcs；HYRC；HYRC1；DNA-dependent Protein kinase catalytic subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P78527
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Expression Region
3746-4128aa
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AA Sequence
REHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVP MTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTK MSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPE AFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHA FGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDP GLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKIC YAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPE SGLSE ETQVKCLMDQATDPNILGRTWEGWEPWM
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Molecular Weight
68 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNA-dependent protein kinase (DNA-PK) is a crucial enzyme involved in the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair. The research on DNA-PK and its recombinant protein has garnered significant attention due to its role in maintaining genomic stability and its implications in cancer biology. DNA-PK consists of a catalytic subunit (DNA-PKcs) and a regulatory component (KU70/KU80 heterodimer), which recognize and bind to broken DNA ends, facilitating the recruitment of additional repair factors. Understanding the structure and function of DNA-PKcs is essential for elucidating the mechanisms of DNA repair and the cellular response to genotoxic stress. Furthermore, aberrations in DNA-PK activity have been linked to various malignancies, making it an attractive target for therapeutic interventions. Recent advancements in recombinant protein technology have enabled the production and purification of DNA-PKcs, allowing researchers to study its biochemical properties, regulatory mechanisms, and interactions with other DNA repair proteins. Insights gained from these studies may lead to the development of novel cancer therapies that enhance the efficacy of radiotherapy and chemotherapy by targeting DNA repair pathways. Thus, the investigation of DNA-PKcs as a recombinant protein is crucial for advancing our understanding of DNA repair mechanisms and providing potential avenues for cancer treatment.
