Analytical Data
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Gene name
RFK
- Application
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Alternative Names
RFK；Riboflavin kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q969G6
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Expression Region
1-155aa
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AA Sequence
MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of recombinant RFK (recombinant riboflavin kinase) has gained significance due to its essential role in the metabolism of riboflavin, which is a crucial vitamin for various biological functions. Riboflavin, or vitamin B2, is pivotal in energy production, cellular respiration, and the metabolism of fats, drugs, and steroids. Deficiencies in this vitamin can lead to a range of health issues, including anemia, skin disorders, and impaired cognitive function. As such, understanding RFK's structure and function at a molecular level can provide insights into its enzymatic mechanisms, potential regulatory pathways, and interactions with other metabolic enzymes. Advances in genetic engineering allow for the production of recombinant RFK in various host systems, facilitating the study of its properties and enhancing our knowledge of riboflavin biosynthesis. Furthermore, characterizing RFK can have implications in biotechnology and pharmaceuticals, offering potential pathways for developing supplements or therapeutic agents targeting riboflavin-dependent processes. Overall, the investigation of recombinant RFK is crucial for elucidating its biological significance and potential applications in health and nutrition.
