Analytical Data
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Gene name
CLNK
- Application
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Alternative Names
CLNK; CLNK_HUMAN; Cytokine-dependent hematopoietic cell linker; Mast cell immunoreceptor signal transducer; MGC159342.; MGC35197; MIST; RGD1562417
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z7G1
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Expression Region
1-428aa
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AA Sequence
MNRQGNRKTTKEGSNDLKFQNFSLPKNRSWPRINSATGQYQRMNKPLLDWERNFAAVLDGAKGHSDDDYDDPELRMEETWQSIKILPARPIKESEYADTHYFKVAMDTPLPLDTRTSISIGQPTWNTQTRLERVDKPISKDVRSQNIKGDASVRKNKIPLPPPRPLITLPKKYQPLPPEPESSRPPLSQRHTFPEVQRMPSQISLRDLSEVLEAEKVPHNQRKPESTHLLENQNTQEIPLAISSSSFTTSNHSVQNRDHRGGMQPCSPQRCQPPASCSPHENILPYKYTSWRPPFPKRSDRKDVQHNEWYIGEYSRQAVEEAFMKENKDGSFLVRDCSTKSKEEPYVLAVFYENKVYNVKIRFLERNQQFALGTGLRGDEKFDSVEDIIEHYKNFPIILIDGKDKTGVHRKQCHLTQPLPLTRHLLPL
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Molecular Weight
76 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CLNK (Cytoplasmic Linker of the Kinase) is a vital adapter protein that plays a key role in immune cell signaling, particularly in the context of lymphocyte activation and proliferation. Its function is primarily associated with the cytoplasmic domain of receptors involved in immune responses, facilitating the propagation of signals from the cell membrane to intracellular pathways. Research has shown that CLNK interacts with various kinases and signaling molecules, such as Syk and ITAM-containing receptors, highlighting its importance in the regulation of immune cell functions, including T-cell and natural killer (NK) cell activities. Dysregulation of CLNK has been implicated in several immunological disorders, including autoimmune diseases and cancers, making it a significant target for therapeutic interventions. The study of CLNK recombinant proteins aims to elucidate its structural and functional mechanisms, enhancing our understanding of immune signaling pathways. By generating and characterizing CLNK proteins, researchers hope to explore its role in cellular responses and potentially uncover novel strategies to modulate immune functions, providing insights that could lead to the development of new treatments for immune-related diseases. This area of research not only contributes to fundamental immunology but also opens avenues for the advancement of biotechnological applications in immunotherapy and vaccine development.
