Analytical Data
-
基因名
bglIIM
- Application
-
别名
bglIIM；Type II methyltransferase M.BglII
-
种属
E.coli
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q45489
-
表达区间
1-360aa
-
氨基酸序列
MSEDQYKQIKLHLGMEDDNEDLPNHIPSSFPKQHLNKIYNGDTMNMLLDIPDNSVDLVVTSPPYNINKFKNDRRPLEEYLKWQTEIIEQCHRVLKPSGSIFWQVGTYVNDSGAHIPLDIRFFPIFESLGMFPRNRIVWVRPHGLHANKKFAGRHETILWFTKTPEYKFFLDPIRVPQKYANKKHYKGDKKGELSGDPLGKNPGDVWAFRNVRHNHEEDTIHPTQYPEDMIERIVLSTTEPNDIVLDPFIGMGTTASVAKNLNRYFYGAEIEKEYVDIAYQILSGEPDENNNFPNLKTLRQYCEKNGIIDPSQYTFTRQRKGSKPSLDSKAHPEEHHKKEIVERIEFEAENSVYKKVQNEQ
-
分子量
58.0 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
BglIIM is a recombinant protein derived from the bacterium Bacillus amyloliquefaciens, which is known for its ability to break down complex carbohydrates. Research on BglIIM has gained significant attention due to its potential applications in various fields, including biotechnology, food industry, and biofuel production. The enzyme is classified as a thermostable glycoside hydrolase, capable of hydrolyzing beta-glucosidic bonds in a wide range of substrates. This property makes it a valuable tool for enhancing the efficiency of biomass conversion processes, such as the production of fermentable sugars from agricultural waste materials. Furthermore, the study of BglIIM's structure and function can provide insights into enzyme mechanisms, making it a subject of interest in molecular biology. Investigating the expression and purification of BglIIM in recombinant systems can improve its availability and cost-effectiveness for industrial applications. Overall, the research into BglIIM not only contributes to fundamental knowledge in enzymology and microbiology but also holds promise for developing innovative solutions for sustainable resource management and alternative energy production.
