Analytical Data
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基因名
GCKR
- Application
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别名
GCKR；Glucokinase regulatory Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q14397
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表达区间
1-625aa
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氨基酸序列
MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIV RLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGG LVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASRE GTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAV FLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIG PEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFE RAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECI HTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSL TEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLK KLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKI LQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSD DIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEA VRSALAGPGQKRTADPLEILEPDVQ
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分子量
85 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GCKR (Glucokinase Regulatory Protein) is an important regulator of glucose metabolism and is associated with the pathways controlling insulin sensitivity and hepatic glucose production. Recent genome-wide association studies (GWAS) have identified several single nucleotide polymorphisms (SNPs) in the GCKR gene that correlate with fasting glucose levels and the risk of type 2 diabetes. This has spurred research interest in GCKR as a therapeutic target for metabolic disorders. Studies have shown that GCKR modulates the activity of glucokinase, a key enzyme in glucose sensing and metabolism. Understanding the functional implications of GCKR variants and their impact on glucose homeostasis is crucial, as it may illuminate new avenues for drug development. The recombinant protein of GCKR has been produced to facilitate in-depth biochemical analyses and to explore its role in glucose regulation, insulin signaling, and lipid metabolism. These investigations aim to unravel the complex interactions between GCKR and other metabolic pathways, which could lead to innovative strategies for preventing and treating metabolic diseases. Moreover, characterizing GCKR's structure and function will enhance our understanding of its regulatory mechanisms and provide insights into how genetic variations may influence individual susceptibility to metabolic disorders, paving the way for personalized medical approaches in diabetes management.
