Analytical Data
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Gene name
SIAE
- Application
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Alternative Names
SIAE；YSG2；Sialate O-acetylesterase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HAT2
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Expression Region
1-523aa
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AA Sequence
MVAPGLVLGLVLPLILWADRSAGIGFRFASYINNDMVLQKEPAGAVIWGF GTPGATVTVTLRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQ QTLEKINFTLRVHDVLFGDVWLCSGQSNMQMTVLQIFNATRELSNTAAYQ SVRILSVSPIQAEQELEDLVAVDLQWSKPTSENLGHGYFKYMSAVCWLFG RHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQGSIPYDSVTG PSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDW RETFHRGSQGQTERFFPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYV PNPKMPNTFMAVAMDLCDRDSPFGSIHPRDKQTVAYRLHLGARALAYGEK NLTFEGPLPEKIELLAHKGLLNLTYYQQIQVQKKDNKIFEISCCSDHRCK WLPASMNTVSTQSLTLAIDSCHGTVVALRYAWTTWPCEYKQCPLYHPSSA LPAPPFIAFITDQGPGHQSNVAK
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SIAE (Sialic Acid Acetylesterase) is an enzyme implicated in the regulation of sialylation, a critical post-translational modification that influences cellular interactions, signaling, and immune responses. Research has demonstrated that SIAE plays a crucial role in modulating the immune system by removing sialic acid residues from glycoproteins and glycolipids, thereby affecting the recognition and clearance of pathogens and apoptotic cells. Dysregulation of SIAE activity has been linked to various diseases, including autoimmune disorders and cancer, where altered sialylation patterns can lead to immune evasion and tumor progression. The study of SIAE recombinant proteins has gained traction due to their potential applications in therapeutic development and as biomarkers for disease diagnosis. By producing SIAE in recombinant form, researchers can investigate its structure-function relationships, enzymatic mechanisms, and interaction with sialylated substrates. This research not only enhances our understanding of SIAE's biological functions but also paves the way for novel interventions targeting sialylation pathways in disease states. Overall, the investigation of SIAE recombinant proteins offers valuable insights into cellular biology and the development of strategies to manipulate the sialylation process for therapeutic benefit.
