Analytical Data
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Gene name
DGUOK
- Application
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Alternative Names
DGUOK；DGK；Deoxyguanosine kinase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16854
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Expression Region
40-277aa
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AA Sequence
RLSIEGNIAVGKSTFVKLLTKTYPEWHVATEPVATWQNIQAAGTQKACTAQSLGNLLDMMYREPARWSYTFQTFSFLSRLKVQLEPFPEKLLQARKPVQIFERSVYSDRYIFAKNLFENGSLSDIEWHIYQDWHSFLLWEFASRITLHGFIYLQASPQVCLKRLYQRAREEEKGIELAYLEQLHGQHEAWLIHKTTKLHFEALMNIPVLVLDVNDDFSEEVTKQEDLMREVNTFVKNL
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Molecular Weight
44.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DGUOK (Deoxyguanosine Kinase) is a crucial enzyme involved in the salvage pathway of purine metabolism, responsible for the phosphorylation of deoxyguanosine to deoxyguanosine monophosphate (dGMP). Mutations or deficiencies in the DGUOK gene can lead to severe mitochondrial diseases characterized by impaired DNA synthesis, oxidative stress, and cellular apoptosis. Understanding the function and structure of DGUOK is essential for deciphering the biochemical pathways impacted by its dysfunction, as well as for developing potential therapeutic strategies for related mitochondrial disorders. Researchers have increasingly focused on the reconstruction and characterization of DGUOK protein to gain insights into its enzymatic activity and to explore the molecular basis of its interactions with substrates and potential inhibitors. Such investigations may also facilitate the design of gene therapy approaches and the development of small molecules that can modulate DGUOK activity, offering hope for patients suffering from DGUOK-related conditions. Overall, the study of DGUOK recombinant protein not only advances our knowledge of mitochondrial biology but also holds promise for innovative treatments for genetic disorders linked to purine metabolism.
