Analytical Data
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Gene name
FAM175B
- Application
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Alternative Names
FAM175B；ABRO1；FAM175B；KIAA0157；BRISC complex subunit Abraxas 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15018
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Expression Region
1-415aa
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AA Sequence
MAASISGYTF SAVCFHSANS NADHEGFLLG EVRQEETFSI SDSQISNTEF LQVIEIHNHQ PCSKLFSFYD YASKVNEESL DRILKDRRKK VIGWYRFRRN TQQQMSYREQ VLHKQLTRIL GVPDLVFLLF SFISTANNST HALEYVLFRP NRRYNQRISL AIPNLGNTSQ QEYKVSSVPN TSQSYAKVIK EHGTDFFDKD GVMKDIRAIY QVYNALQEKV QAVCADVEKS ERVVESCQAE VNKLRRQITQ RKNEKEQERR LQQAVLSRQM PSESLDPAFS PRMPSSGFAA EGRSTLGDAE ASDPPPPYSD FHPNNQESTL SHSRMERSVF MPRPQAVGSS NYASTSAGLK YPGSGADLPP PQRAAGDSGE DSDDSDYENL IDPTEPSNSE YSHSKDSRPM AHPDEDPRNT QTSQI
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Molecular Weight
46.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FAM175B, also known as ABRAXAS2, is a crucial component associated with the BRCA1-A complex involved in DNA damage response and repair mechanisms. The study of recombinant FAM175B protein has gained prominence due to its potential implications in cancer biology, particularly in the context of breast and ovarian cancers linked to BRCA1 mutations. Understanding the structure and function of FAM175B is vital for elucidating its role in maintaining genomic stability and how its dysregulation may lead to tumorigenesis. Recombinant protein expression systems facilitate the production of FAM175B, allowing researchers to study its biochemical properties and interactions. By utilizing techniques such as X-ray crystallography and NMR spectroscopy, insights into the protein's 3D structure can be gained, which aids in the identification of potential drug targets that could restore normal BRCA1 function in cancer cells. Additionally, examining the post-translational modifications of FAM175B can provide further understanding of its regulatory mechanisms in cellular contexts. Overall, the research on recombinant FAM175B protein is essential for developing therapeutic strategies that could mitigate the effects of BRCA1-associated cancers and contribute to the advancement of precision medicine in oncology.
