Analytical Data
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基因名
BTD
- Application
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别名
BTD；Biotinidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P43251-3
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表达区间
44-545aa
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氨基酸序列
AHTGEESVADHHEAEYYVAAVYEHPSILSLNPLALISRQEALELMNQNLD IYEQQVMTAAQKDVQIIVFPEDGIHGFNFTRTSIYPFLDFMPSPQVVRWN PCLEPHRFNDTEVLQRLSCMAIRGDMFLVANLGTKEPCHSSDPRCPKDGR YQFNTNVVFSNNGTLVDRYRKHNLYFEAAFDVPLKVDLITFDTPFAGRFG IFTCFDILFFDPAIRVLRDYKVKHVVYPTAWMNQLPLLAAIEIQKAFAVA FGINVLAANVHHPVLGMTGSGIHTPLESFWYHDMENPKSHLIIAQVAKNP VGLIGAENATGETDPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAP PTFHSEMMYDNFTLVPVWGKEGYLHVCSNGLCCYLLYERPTLSKELYALG VFDGLHTVHGTYYIQVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNFS TSYIFPLFLTSGMTLEVPDQLGWENDHYFLRKSRLSSGLVTAALYGRLYE RDLEHHHHHH
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分子量
58 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BTD (Brachyury) is a T-box transcription factor crucial for mesoderm formation and notochord development in various vertebrate species. Its pivotal role in early embryonic processes has garnered significant attention in developmental biology and regenerative medicine. BTD is also implicated in several pathological conditions, including cancer, where it may contribute to tumor progression and metastasis by influencing cell differentiation and survival. Research on BTD recombinant proteins aims to elucidate their structural and functional characteristics, providing insights into their molecular mechanisms and potential therapeutic applications. By producing BTD as a recombinant protein, scientists can conduct detailed studies to explore its interaction with target genes and proteins, paving the way for advanced treatments targeting BTD-related pathways. This research not only helps in understanding developmental processes but also offers the potential for novel cancer therapies, underscoring the importance of BTD in both developmental biology and clinical research.
