Analytical Data
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Gene name
SCN5A
- Application
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Alternative Names
Cardiac tetrodotoxin insensitive voltage dependent sodium channel alpha subunit; CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; ICCD; IVF; LQT3; PFHB1; Scn5a (gene name); Scn5a; SCN5A_HUMAN; Sodium channel protein cardiac muscle alpha subunit; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type 5 subunit alpha; Sodium channel protein type V alpha subunit; Sodium channel protein type V subunit alpha; SSS1; VF1; Voltage gated sodium channel alpha subunit Nav1.5; Voltage-gated sodium channel subunit alpha Nav1.5
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14524
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Expression Region
1-223 aa
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AA Sequence
MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAASVLGTLFFPMSIQATSTS
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Molecular Weight
51.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SCN5A is a gene that encodes the alpha subunit of the voltage-gated sodium channel NaV1.5, which is predominantly expressed in cardiac tissue. Research on SCN5A is pivotal due to its critical role in cardiac excitability and conduction. Mutations in the SCN5A gene are associated with various cardiac arrhythmias, such as Long QT Syndrome, Brugada Syndrome, and other inherited channelopathies. These mutations can lead to altered ion channel function, resulting in impaired electrical signaling in the heart. Therefore, the study of SCN5A recombinant proteins has become increasingly important for understanding the mechanisms underlying these diseases. By expressing and analyzing SCN5A recombinant proteins in vitro, researchers can investigate the effects of specific mutations on the channel's biophysical properties, pharmacological responses, and overall cellular function. This work aids in elucidating the pathophysiology of associated arrhythmias, developing potential therapeutic strategies, and advancing personalized medicine approaches for affected patients. Enhanced understanding through SCN5A recombinant protein studies may also contribute to the development of novel drugs targeting sodium channels, aiming to restore normal cardiac function in individuals with SCN5A-related conditions.
