Analytical Data
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Gene name
CHML
- Application
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Alternative Names
CHM like; Chml; Choroideraemia like Protein; Choroideraemia-like Protein; Choroideremia like (Rab escort Protein 2); Choroideremia like; Choroideremia like Protein; Rab escort Protein 2; Rab Proteins geranylgeranyltransferase component A 2; RAE2_HUMAN; REP 2; REP-2; REP2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P26374
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Expression Region
1-656aa
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AA Sequence
MADNLPTEFDVVIIGTGLPESILAAACSRSGQRVLHIDSRSYYGGNWASFSFSGLLSWLKEYQQNNDIGEESTVVWQDLIHETEEAITLRKKDETIQHTEAFCYASQDMEDNVEEIGALQKNPSLGVSNTFTEVLDSALPEESQLSYFNSDEMPAKHTQKSDTEISLEVTDVEESVEKEKYCGDKTCMHTVSDKDGDKDESKSTVEDKADEPIRNRITYSQIVKEGRRFNIDLVSKLLYSQGLLIDLLIKSDVSRYVEFKNVTRILAFREGKVEQVPCSRADVFNSKELTMVEKRMLMKFLTFCLEYEQHPDEYQAFRQCSFSEYLKTKKLTPNLQHFVLHSIAMTSESSCTTIDGLNATKNFLQCLGRFGNTPFLFPLYGQGEIPQGFCRMCAVFGGIYCLRHKVQCFVVDKESGRCKAIIDHFGQRINAKYFIVEDSYLSEETCSNVQYKQISRAVLITDQSILKTDLDQQTSILIVPPAEPGACAVRVTELCSSTMTCMKDTYLVHLTCSSSKTAREDLESVVKKLFTPYTETEINEEELTKPRLLWALYFNMRDSSGISRSSYNGLPSNVYVCSGPDCGLGNEHAVKQAETLFQEIFPTEEFCPPPPNPEDIIFDGDDKQPEAPGTNNVVMAKLESSEESKNLESPEKHLQN
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Molecular Weight
100.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of CHML (Chaperonin-Containing Tetradecamer) recombinant protein has gained significant attention in the fields of molecular biology and biochemistry due to its crucial role in protein folding and cellular stress responses. CHML is a member of the chaperonin family, which assists in the proper folding of nascent polypeptides, thereby ensuring the correct functioning of proteins essential for various cellular processes. Abnormalities in protein folding can lead to diseases, including neurodegenerative disorders and certain cancers, making CHML a potential target for therapeutic interventions. Recent advancements in recombinant DNA technology have enabled researchers to produce large quantities of CHML, facilitating detailed structural and functional studies. Investigating the properties of CHML recombinant protein enhances our understanding of its mechanism of action and its interactions with other cellular components. Furthermore, these studies provide insights into the evolution of chaperonin systems across different organisms, highlighting their conserved roles in maintaining proteostasis. Overall, the exploration of CHML recombinant protein not only fills gaps in our knowledge regarding cellular physiology but also paves the way for innovative approaches in drug development and therapeutic strategies for mitigating diseases associated with protein misfolding.
