Analytical Data
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Gene name
RBMY1F
- Application
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Alternative Names
RBMY1F；YRRM2；RBMY1J；RNA-binding motif Protein. Y chromosome. family 1 member F/J
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q15415
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Expression Region
1-496aa
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AA Sequence
MVEADHPGKLFIGGLNRETNEKMLKAVFGKHGPISEVLLIKDRTSKSRGFAFITFENPADAKNAAKDMNGTSLHGKAIKVEQAKKPSFQSGGRRRPPASSRNRSPSGSLRSARGSSGGTRGWLPSHEGHLDDGGYTPDLKMSYSRGLIPVKRGPSSRSGGPPPKKSAPSAVARSNSWMGSQGPMSQRRENYGVPPRRATISSWRNDRMSTRHDGYATNDGNHPSCQETRDYAPPSRGYAYRDNGHSNRDEHSSRGYRNHRSSRETRDYAPPSRGHAYRDYGHSRRDESYSRGYRNHRSSRETREYAPPSRGHGYRDYGHSRRHESYSRGYRNHPSSRETRDYAPPHRDYAYRDYGHSSWDEHSSRGYSYHDGYGEALGRDHSEHLSGSSYRDALQRYGTSHGAPPARGPRMSYGGSTCHAYSNTRDRYGRSWESYSSCGDFHYCDREHVCRKDQRNPPSLGRVLPDPREAYGSSSYVASIVDGGESRSEKGDSSRY
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Molecular Weight
71.7kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBMY1F, a member of the RNA-binding motif protein family, plays a significant role in the regulation of gene expression, particularly during spermatogenesis. This protein is encoded by the RBMY1 gene located on the Y chromosome, which highlights its importance in male fertility and the development of germ cells. Research has shown that RBMY1F is involved in the post-transcriptional regulation of target mRNAs, influencing processes such as mRNA stability, translational control, and splicing. Given its pivotal role in male reproductive biology, mutations or alterations in RBMY1F expression can lead to infertility and other reproductive disorders. The study of RBMY1F as a recombinant protein offers insights into its functional mechanisms and potential applications in reproductive medicine. Moreover, understanding its interactions with various RNA targets may reveal novel therapeutic approaches for treating infertility associated with Y chromosome anomalies. Thus, the research surrounding RBMY1F is not only fundamental for elucidating the complexities of male fertility but also holds significant potential for clinical implications in reproductive health.
