Analytical Data
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基因名
XRCC4
- Application
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别名
XRCC4；DNA repair Protein XRCC4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13426
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表达区间
1-336aa
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氨基酸序列
MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
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分子量
65.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
XRCC4 (X-ray repair cross-complementing protein 4) is a crucial component of the non-homologous end joining (NHEJ) pathway, which is a primary mechanism for repairing DNA double-strand breaks. Dysfunction or mutations in the XRCC4 gene can lead to significant genomic instability and contribute to various diseases, including cancer. Research on XRCC4 recombinant protein is pivotal for understanding its role in maintaining genomic integrity and its interactions with other proteins involved in the DNA repair process. By studying XRCC4, scientists aim to elucidate its structural and functional characteristics, which may reveal how it facilitates the assembly and stabilization of repair complexes at DNA break sites. Additionally, the development of XRCC4 recombinant protein provides a valuable tool for biochemical assays, enabling in-depth investigation of the mechanistic details of NHEJ, as well as potential therapeutic targets for enhancing DNA repair in disease contexts. The exploration of XRCC4 also extends to its implications in cellular responses to ionizing radiation and other forms of DNA damage, making it a focal point in both cancer biology and therapeutic development. Thus, the study of XRCC4 recombinant protein not only furthers our understanding of fundamental molecular biology but also opens avenues for novel cancer therapies and interventions in genomic repair mechanisms.
