Analytical Data
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Gene name
CHD2
- Application
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Alternative Names
2810013C04Rik; 2810040A01Rik; 5630401D06Rik; AI851092; ATP dependent helicase CHD2; ATP-dependent helicase CHD2; BC029703; CHD 2; CHD-2; CHD2; CHD2_HUMAN
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Species
Human
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Source
E. coli
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Tag
GST tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14647
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Expression Region
260-456
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AA Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSETIEKVLDSRLGKKGATG ASTTVYAIEANGDPSGDFDTEKDEGEIQYLIKWKGWSYIHSTWESEESLQ QQKVKGLKKLENFKKKEDEIKQWLGKVSPEDVEYFNCQQELASELNKQYQ IVERVIAVKTSKSTLGQTDFPAHSRKPAPSNEPEYLCKWMGLPYSECSWE DEALIGKKFQNCIDSFHSRNNSKTIPTR
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Molecular Weight
49 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHD2 (Chromodomain Helicase DNA Binding Protein 2) is a member of the CHD protein family, which plays a crucial role in chromatin remodeling and regulation of gene expression. It contains chromodomains that are involved in recognizing methylated lysines on histones, linking it to epigenetic regulation. Recent studies have highlighted CHD2's essential functions in maintaining genome stability, controlling DNA repair processes, and influencing cell differentiation and development. Furthermore, mutations in the CHD2 gene have been associated with various neurological disorders, underscoring its significance in human health. The investigation of recombinant CHD2 protein is pivotal for understanding its functional mechanisms, interactions with DNA and other proteins, and the pathways it regulates. By producing and analyzing recombinant CHD2, researchers aim to elucidate its role in chromatin dynamics and its implications in disease, thereby advancing the potential for targeted therapies. This research could also enhance our understanding of fundamental biological processes, such as transcription regulation and cellular response to DNA damage, contributing to the broader field of epigenetics.
