Analytical Data
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Gene name
SAV1
- Application
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Alternative Names
1700040G09Rik; 45 kDa WW domain protein; hWW 45; hWW45; Protein salvador homolog 1; salvador family WW domain containing protein 1; salvador homolog 1 (Drosophila); Salvador homolog 1; Salvador. Drosophila. homolog of; SAV 1; SAV; SAV1; SAV1_HUMAN; WW 45; WW domain containing; WW domain-containing adaptor 45; WW domain-containing protein. 45-KD; WW45; WW45 protein; WWP 4; WWP4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H4B6
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Expression Region
1-383 aa
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AA Sequence
MLSRKKTKNE VSKPAEVQGK YVKKETSPLL RNLMPSFIRH GPTIPRRTDI CLPDSSPNAF STSGDVVSRN QSFLRTPIQR TPHEIMRRES NRLSAPSYLA RSLADVPREY GSSQSFVTEV SFAVENGDSG SRYYYSDNFF DGQRKRPLGD RAHEDYRYYE YNHDLFQRMP QNQGRHASGI GRVAATSLGN LTNHGSEDLP LPPGWSVDWT MRGRKYYIDH NTNTTHWSHP LEREGLPPGW ERVESSEFGT YYVDHTNKKA QYRHPCAPSV PRYDQPPPVT YQPQQTERNQ SLLVPANPYH TAEIPDWLQV YARAPVKYDH ILKWELFQLA DLDTYQGMLK LLFMKELEQI VKMYEAYRQA LLTELENRKQ RQQWYAQQHG KNF
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Molecular Weight
44.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SAV1 (Salvador homolog 1) is a crucial component of the Hippo signaling pathway, which plays a pivotal role in regulating cell proliferation, apoptosis, and tissue homeostasis. The Hippo pathway is essential for controlling organ size and preventing tumorigenesis, making SAV1 a significant focus of cancer research and developmental biology. Recent studies have revealed that SAV1 acts as a scaffolding protein that facilitates the interaction between various kinases in the Hippo pathway, thereby influencing its downstream effects on the transcriptional coactivators YAP and TAZ. This regulation is vital for maintaining normal cellular functions and preventing excessive growth. Given its importance, researchers have been investigating the structure and function of SAV1 through recombinant protein techniques. These studies aim to elucidate the molecular mechanisms by which SAV1 contributes to Hippo pathway signaling and to explore its potential as a therapeutic target for cancer treatment. The recombinant production of SAV1 not only provides an opportunity to examine its biochemical properties but also facilitates high-throughput screening of drugs that may modulate its activity. Understanding the complex roles of SAV1 in various cellular contexts could lead to innovative strategies for cancer therapies and regenerative medicine, highlighting its relevance in both basic and applied scientific research.
