Cat: PA2000-3008

Recombinant E.coli pmpD Protein,His

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Analytical Data

  • Gene name

    pmpD

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    pmpD;Probable outer membrane Protein PmpD

  • Species

    E.coli

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    O84818

  • Expression Region

    1244-1531aa

  • AA Sequence

    EFDYSTNVWGFAFGGFRTLSAENLVAIDGYKGAYGGASAGVDIQLMEDFVLGVSGAAFLGKMDSQKFDAEVSRKGVVGSVYTGFLAGSWFFKGQYSLGETQNDMKTRYGVLGESSASWTSRGVLADALVEYRSLVGPVRPTFYALHFNPYVEVSYASMKFPGFTEQGREARSFEDASLTNITIPLGMKFELAFIKGQFSEVNSLGISYAWEAYRKVEGGAVQLLEAGFDWEGAPMDLPRQELRVALENNTEWSSYFSTVLGLTAFCGGFTSTDSKLGYEANTGLRLIF

  • Molecular Weight

    47.5 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

PmpD is a protein implicated in the pathogenesis of various bacterial infections, particularly those caused by Mycobacterium tuberculosis, which remains a significant global health challenge. This recombinant protein has garnered attention due to its potential role as an immunogen in vaccine development. Studies suggest that PmpD may play a crucial part in bacterial virulence and immune evasion, making it an attractive target for therapeutic interventions. The understanding of PmpD's structure and function is essential for elucidating its role in the immune response and its interactions with host cells. Consequently, the recombinant expression of PmpD allows researchers to study its immunological properties and assess its potential use in diagnostic tools and vaccines. Investigations into PmpD's characteristics contribute to the broader understanding of mycobacterial biology and may lead to innovative strategies for combating tuberculosis and related diseases. Overall, the study of PmpD and its recombinant forms is pivotal in addressing the pressing need for effective vaccines and treatments against persistent bacterial infections.

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