Analytical Data
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基因名
CENTB1
- Application
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别名
Arf-GAP with coiled-coil. ANK repeat and PH domain-containing Protein 1. Centaurin-beta-1. Cnt-b1
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15027
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表达区间
1-740aa
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氨基酸序列
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL
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分子量
107.9 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CENTB1 (Centromere Protein B1) is a protein that has garnered attention in the field of cellular biology due to its critical role in cell division and its involvement in centromere function. Centromeres are essential for the proper segregation of chromosomes during mitosis and meiosis, and any dysfunction in centromere proteins like CENTB1 can lead to chromosomal instability, which is often implicated in various diseases, including cancer. Recent studies have highlighted the importance of CENTB1 in the assembly and maintenance of the kinetochore, a protein complex that mediates chromosome movement. Furthermore, research has shown that CENTB1's interactions with other centromere-associated proteins contribute to the correct positioning and stability of chromosomes during cell division. Understanding the structure and function of CENTB1 through methods such as recombinant protein expression and purification has become crucial for exploring its biochemical properties and mechanisms of action. Investigating CENTB1's role not only enhances our knowledge of cellular processes but also opens potential avenues for therapeutic targeting in diseases characterized by chromosomal abnormalities. As research progresses, CENTB1 continues to be a significant focus for scientists aiming to unravel the complexities of cell cycle regulation and genomic integrity.
