Analytical Data
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Gene name
S1PR4
- Application
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Alternative Names
Sphingosine 1-phosphate receptor 4. S1P receptor 4. S1P4. Endothelial differentiation G-protein coupled receptor 6. Sphingosine 1-phosphate receptor Edg-6. S1P receptor Edg-6
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95977
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AA Sequence
MNATGTPVAPESCQQLAAGGHSRLIVLHYNHSGRLAGRGGPEDGGLGALRGLSVAASCLVVLENLLVLAAITSHMRSRRWVYYCLVNITLSDLLTGAAYLANVLLSGARTFRLAPAQWFLREGLLFTALAASTFSLLFTAGERFATMVRPVAESGATKTSRVYGFIGLCWLLAALLGMLPLLGWNCLCAFDRCSSLLPLYSKRYILFCLVIFAGVLATIMGLYGAIFRLVQASGQKAPRPAARRKARRLLKTVLMILLAFLVCWGPLFGLLLADVFGSNLWAQEYLRGMDWILALAVLNSAVNPIIYSFRSREVCRAVLSFLCCGCLRLGMRGPGDCLARAVEAHSGASTTDSSLRPRDSFRGSRSLSFRMREPLSSISSVRSI
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Molecular Weight
41.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
S1PR4, or sphingosine-1-phosphate receptor 4, is a member of the sphingosine-1-phosphate receptor family, which are G protein-coupled receptors (GPCRs) that play critical roles in various physiological and pathological processes. The study of S1PR4 has gained attention due to its involvement in immune regulation, vascular biology, and neuroprotection. In particular, S1PR4 is known to modulate the trafficking and activation of various immune cells, influencing inflammatory responses and maintaining immune homeostasis. Several studies have indicated that S1PR4 can have both pro-inflammatory and anti-inflammatory effects depending on the context, making it a potential target for therapeutic interventions in autoimmune diseases, cardiovascular disorders, and cancer. The development and characterization of recombinant S1PR4 proteins have facilitated research into the receptor's structure-function relationships and its signaling pathways. Such studies are essential for elucidating the receptor's biological roles and for designing specific modulators that could lead to new treatments. The recombinant protein can be utilized in binding assays, receptor localization studies, and high-throughput screening of compounds that might affect its activity. Understanding S1PR4’s molecular mechanisms not only provides insights into its function in health and disease but also underscores its potential as a biomarker and therapeutic target in various medical conditions. Thus, the ongoing research on S1PR4 and its recombinant protein underscores its relevance in pharmacology and clinical research, paving the way for novel strategies in drug development and targeted therapies.
