Analytical Data
-
Gene name
CCT4
- Application
-
Alternative Names
CCT4; CCTD; SRB; T-complex Protein 1 subunit delta; TCP-1-delta; CCT-delta; Stimulator of TAR RNA-binding
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P50991
-
Expression Region
1-539aa
-
AA Sequence
MPENVAPRSGATAGAAGGRGKGAYQDRDKPAQIRFSNISAAKAVADAIRTSLGPKGMDKMIQDGKGDVTITNDGATILKQMQVLHPAARMLVELSKAQDIEAGDGTTSVVIIAGSLLDSCTKLLQKGIHPTIISESFQKALEKGIEILTDMSRPVELSDRETLLNSATTSLNSKVVSQYSSLLSPMSVNAVMKVIDPATATSVDLRDIKIVKKLGGTIDDCELVEGLVLTQKVSNSGITRVEKAKIGLIQFCLSAPKTDMDNQIVVSDYAQMDRVLREERAYILNLVKQIKKTGCNVLLIQKSILRDALSDLALHFLNKMKIMVIKDIEREDIEFICKTIGTKPVAHIDQFTADMLGSAELAEEVNLNGSGKLLKITGCASPGKTVTIVVRGSNKLVIEEAERSIHDALCVIRCLVKKRALIAGGGAPEIELALRLTEYSRTLSGMESYCVRAFADAMEVIPSTLAENAGLNPISTVTELRNRHAQGEKTAGINVRKGGISNILEELVVQPLLVSVSALTLATETVRSILKIDDVVNTR
-
Molecular Weight
84.3 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CCT4, a member of the chaperonin-containing TCP1 (CCT) complex, plays a crucial role in the proper folding of various cytosolic proteins, particularly those involved in cellular processes such as cytoskeletal dynamics, cell cycle regulation, and signal transduction. The CCT complex is essential for the maintenance of protein homeostasis, and its dysfunction can lead to various diseases, including neurodegenerative disorders and cancer. Research on CCT4 has gained prominence due to its unique properties and interactions within the CCT complex, which facilitate the folding of actin and tubulin, key components of the cytoskeleton. Additionally, CCT4's involvement in the assembly of multi-protein complexes further emphasizes its significance in cellular function and development. Investigating CCT4 has implications for understanding the molecular mechanisms underlying protein misfolding diseases and identifying potential therapeutic targets. The study of CCT4 recombinant protein has thus become a focal point for researchers aiming to unravel the intricate details of protein folding pathways and their association with human health. Overall, the exploration of CCT4 not only enhances our understanding of chaperonin biology but also provides insight into the broader implications of protein homeostasis in disease and development.
