Analytical Data
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Gene name
FLP1
- Application
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Alternative Names
FLP1；Flp1；Ptpk1；Tyrosine-Protein phosphatase non-receptor type 18
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Species
Saccharomyces cerevisiae
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P03870
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Expression Region
1-423aa
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AA Sequence
MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI
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Molecular Weight
54.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FLP1 recombinase is a crucial enzyme derived from the yeast *Saccharomyces cerevisiae*, known for its ability to catalyze site-specific DNA recombination. This enzyme belongs to the integrase family and operates through a well-characterized mechanism involving the recognition of specific DNA sequences, leading to excision, inversion, or integration of DNA segments. Its utility has expanded significantly in diverse fields, including molecular biology, genetics, and biotechnology. Researchers employ FLP1 recombinase as a tool for gene targeting, transgenic animal creation, and conditional gene expression, facilitating the study of gene function and regulation. The development of FLP/FRT (FLP recombinase target) systems has revolutionized genetic manipulation, enabling precise control over gene expression in various organisms. Recent advances in synthetic biology and genome editing have further highlighted the importance of FLP1 in creating novel genetic constructs and pathways. Understanding the structural and functional characteristics of FLP1 is essential for optimizing its application in experimental systems and therapeutic contexts, including gene therapy and the production of genetically modified organisms. Thus, ongoing research focuses on elucidating the mechanism of FLP1 action, improving its efficiency, and expanding its versatility as a genetic engineering tool.
