Analytical Data
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Gene name
NEIL1
- Application
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Alternative Names
NEIL1；Endonuclease 8-like 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96FI4
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Expression Region
1-390aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NEIL1 (Nth Enzyme Involved in DNA Repair 1) is a significant protein in the field of DNA repair and cellular stress response. It belongs to the DNA glycosylase family, which plays a crucial role in recognizing and excising damaged bases in DNA, particularly those resulting from oxidative stress. The importance of NEIL1 arises from its capacity to repair oxidative lesions such as 8-oxoguanine and other modified bases generated by reactive oxygen species. These lesions, if left unrepaired, can lead to mutations, genomic instability, and contribute to various pathological conditions, including cancer and neurodegenerative diseases. Research on NEIL1 involves understanding its structural biology, enzymatic mechanisms, and regulatory pathways, which are essential for maintaining genomic integrity. Furthermore, studies have revealed that NEIL1 interacts with other DNA repair factors, indicating its cooperative role in the broader DNA repair network. Investigating NEIL1's functional significance and its potential as a therapeutic target could provide insights into novel strategies for cancer treatment and prevention, enhancing our understanding of cellular responses to oxidative stress. The recombinant production of NEIL1 allows for in-depth studies of its functional properties and interactions, facilitating the exploration of its roles in health and disease.
