Analytical Data
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Gene name
RNF166
- Application
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Alternative Names
1110031E24Rik; AW555115; MGC14381; MGC2647; RING finger protein 166; RN166_HUMAN; RNF 166; RNF166; Zfp313l; Zinc finger protein 228; Zinc finger protein 313
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96A37
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Expression Region
1-237 aa
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AA Sequence
MAMFRSLVAS AQQRQPPAGP AGGDSGLEAQ YTCPICLEVY HRPVAIGSCG HTFCGECLQP CLQVPSPLCP LCRLPFDPKK VDKATHVEKQ LSSYKAPCRG CNKKVTLAKM RVHISSCLKV QEQMANCPKF VPVVPTSQPI PSNIPNRSTF ACPYCGARNL DQQELVKHCV ESHRSDPNRV VCPICSAMPW GDPSYKSANF LQHLLHRHKF SYDTFVDYSI DEEAAFQAAL ALSLSEN
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Molecular Weight
26.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF166, a member of the RING-type E3 ubiquitin ligase family, has garnered attention due to its potential role in regulating various cellular processes, including protein degradation, cell signaling, and stress responses. Research suggests that RNF166 may be involved in the modulation of key signaling pathways, influencing cell proliferation and apoptosis. Its expression has been linked to various physiological and pathological conditions, including cancer progression and neurodegenerative diseases. The study of RNF166 recombinant protein is crucial for elucidating its functional mechanisms, as well as its interactions with substrates and other cellular proteins. By employing techniques such as gene cloning, expression analysis, and functional assays, researchers aim to dissect the molecular roles of RNF166 in cellular homeostasis and disease states. Understanding the biochemical properties and functional implications of RNF166 can pave the way for novel therapeutic strategies targeting its pathways, offering potential insights into managing diseases associated with aberrant ubiquitination processes. Overall, RNF166 represents a significant research target in the field of molecular biology, with implications for developing innovative treatments for conditions linked to dysregulated protein homeostasis.
