Analytical Data
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Gene name
RNF157
- Application
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Alternative Names
RNF157; KIAA1917; E3 ubiquitin ligase RNF157; EC 2.3.2.27; RING finger protein 157; RING-type E3 ubiquitin transferase RNF157
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96PX1
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Expression Region
2-679 aa
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AA Sequence
GALTSRQHA GVEEVDIPSN SVYRYPPKSG SYFASHFIMG GEKFDSTHPE GYLFGENSDL NFLGNRPVVF PYAAPPPQEP VKTLRSLVNI RKDTLRLVKC AEEVKSPGEE ASKAKVHYNV EFTFDTDARV AITIYYQATE EFQNGIASYI PKDNSLQSET VQYKRGVCQQ FCLPSHTVDP SEWAEEELGF DLDREVYPLV VHAVVDEGDE YFGHCHVLLG TFEKHTDGTF CVKPLKQKQV VDGVSYLLQE IYGIENKYNT QDSKVAEDEV SDNSAECVVC LSDVRDTLIL PCRHLCLCNT CADTLRYQAN NCPICRLPFR ALLQIRAMRK KLGPLSPTSF NPIISSQTSD SEEHPSSENI PPGYEVVSLL EALNGPLTPS PAVPPLHVLG DGHLSGMLPS YGSDGHLPPV RTISPLDRLS DSSSQGLKLK KSLSKSTSQN SSVLHEEEDE HSCSESETQL SQRPSVQHLG EECGVTPESE NLTLSSSGAI DQSSCTGTPL SSTISSPEGP ASSSLAQSVM SMASSQISTD TVSSMSGSYI APGTEEEGEA LSSPQPASRA PSEEGEGLPA ESPDSNFAGL PAGEQDAEGN DVIEEEDGSP TQEGQRTCAF LGMECDNNND FDIASVKALD NKLCSEVCLP GAWQADDNAV SRNAQRRRLS SSSLEDSETR PCVWGPLAV
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Molecular Weight
73.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RNF157 is a protein that belongs to the RING finger E3 ubiquitin ligase family, which plays a crucial role in regulating protein degradation and cellular processes through the ubiquitin-proteasome pathway. The significance of RNF157 in various biological processes, including immune response, cell cycle regulation, and stress responses, has drawn attention in recent years. Mutations or dysregulation of RNF157 have been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Recent studies have focused on the functional characterization of RNF157, revealing its interactions with key signaling pathways and substrates involved in cellular homeostasis. The development of recombinant RNF157 protein allows researchers to investigate its biochemical properties and active mechanisms in detail. By expressing and purifying RNF157 in a suitable system, scientists can conduct assays to explore its activity, stability, and interaction with other molecules. Understanding the structural and functional aspects of RNF157 will not only provide insights into its role in disease mechanisms but also aid in the design of small-molecule inhibitors or modulators that could be beneficial in therapeutic applications. Therefore, the study of RNF157 and its recombinant form is a promising avenue for advancing our knowledge of ubiquitin ligases and their potential implications in health and disease.
