Analytical Data
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Gene name
RNF12
- Application
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Alternative Names
E3 ubiquitin-protein ligase RLIM; LIM domain-interacting RING finger protein; R-LIM; Renal carcinoma antigen NY-REN-43; RING finger LIM domain-binding protein; RING finger protein 12; RLIM; RNF12; RNF12_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NVW2
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Expression Region
1-624 aa
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AA Sequence
MENSDSNDKG SGDQSAAQRR SQMDRLDREE AFYQFVNNLS EEDYRLMRDN NLLGTPGEST EEELLRRLQQ IKEGPPPQNS DENRGGDSSD DVSNGDSIID WLNSVRQTGN TTRSGQRGNQ SWRAVSRTNP NSGDFRFSLE INVNRNNGSQ NSENENEPSA RRSSGENVEN NSQRQVENPR SESTSARPSR SERNSTEALT EVPPTRGQRR ARSRSPDHRR TRARAERSRS PLHPMSEIPR RSHHSISSQT FEHPLVNETE GSSRTRHHVT LRQQISGPEL LSRGLFAASG TRNASQGAGS SDTAASGEST GSGQRPPTIV LDLQVRRVRP GEYRQRDSIA SRTRSRSQTP NNTVTYESER GGFRRTFSRS ERAGVRTYVS TIRIPIRRIL NTGLSETTSV AIQTMLRQIM TGFGELSYFM YSDSDSEPTG SVSNRNMERA ESRSGRGGSG GGSSSGSSSS SSSSSSSSSS SSSSSSPSSS SGGESSETSS DLFEGSNEGS SSSGSSGARR EGRHRAPVTF DESGSLPFLS LAQFFLLNED DDDQPRGLTK EQIDNLAMRS FGENDALKTC SVCITEYTEG NKLRKLPCSH EYHVHCIDRW LSENSTCPIC RRAVLASGNR ESVV
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Molecular Weight
68.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RNF12, also known as RING Finger Protein 12, is an E3 ubiquitin ligase that plays a critical role in various cellular processes, including gene regulation, cell differentiation, and response to stress. Its involvement in the ubiquitin-proteasome system makes it a key player in protein degradation and turnover, which are essential for maintaining cellular homeostasis. Research has indicated that RNF12 is associated with several biological processes and is implicated in various diseases, including cancer and genetic disorders. Given its pivotal role, the study of RNF12 as a recombinant protein is particularly valuable for elucidating its function, interactions, and molecular mechanisms. Researchers aim to produce RNF12 in a controlled laboratory setting to analyze its structure and activity, allowing for a better understanding of its role in ubiquitination pathways and potential therapeutic implications. The ability to generate RNF12 as a recombinant protein can also facilitate the development of targeted interventions in diseases where RNF12 is dysregulated, paving the way for novel treatment strategies. Overall, the investigation of RNF12 recombinant protein not only enhances our understanding of fundamental biological processes but also provides insights that could lead to breakthroughs in medical research and therapeutic development.
