Analytical Data
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Gene name
coaE
- Application
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Alternative Names
coaE；Dephospho-CoA kinase
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A6I9
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Expression Region
1-206aa
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AA Sequence
MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGAPALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQATREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP
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Molecular Weight
42.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of coaE recombinant proteins is rooted in the growing interest in understanding various biochemical pathways and their implications in health and disease. CoaE, or phosphopantetheine adenylyltransferase, plays a crucial role in the biosynthesis of coenzyme A (CoA), a vital cofactor involved in numerous metabolic processes, including lipid metabolism, the citric acid cycle, and the synthesis and degradation of fatty acids. Impairments in CoA metabolism have been linked to several metabolic disorders, making the research on coaE and its recombinant protein version particularly relevant. As scientists aim to elucidate the detailed structure-function relationships of coaE, recombinant proteins are produced to study their enzymatic activity, stability, and interaction with other biomolecules. Additionally, the production of coaE recombinant proteins offers significant potential for therapeutic applications, including the development of enzyme inhibitors that could serve as novel treatments for metabolic syndromes. Overall, studying coaE recombinant proteins contributes to a broader understanding of metabolic regulation and paves the way for innovative therapeutic strategies against related diseases.
