Cat: PA2000-6488

Recombinant Human CCDC148 Protein,His

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Analytical Data

  • Gene name

    CCDC148

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    CCDC148Coiled-coil domain-containing Protein 148

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8NFR7

  • Expression Region

    1-591aa

  • AA Sequence

    MCAASASPDN LVFHMKNEMR NIKYKPVDYQ QLRALTEAKK LASASAKLKI RKAMLTSKLS KEQTLIKQHK QVWWQEYQRL NEVRCKMESE IKSLLNEENI GNECLCDLTN FEQELSEQQC TYLKNVINPI QQLRADLKYR QHHTLQHSHP HIEFNSMKVL EEVDFVKKQL KTVFERLRLE QQRIENDLSD WSIKILDHSL EEKTNPLSEL PIELESLECP YPDLKSSILS EFYKFTQKYQ KKLQDFNLQL EDIYRNCQLS EEDHWIYQAI LDQYPGDLFG RRTLYLDMLQ RYFPHKSRHD LVEHEKYCDQ YRFAIEQQNI LISNWNKNKK DFIQKAVLTL TEACATHEME SMLAKDKKKQ QELCADLKAK VRQWRAHQEE VARLEMEISA RRREKEEEKE KLWKKKELLQ RAEKKKKIKK YWAKKKQKWQ EMEMRDLQRL EELKKLIAEQ SLKDRERVKY RQELLERRLM EKKEVALQEA HEDKERARRL EALRKQVAVV AQFDPVRMMS DTMASKARMG IEIEEEFILQ KPLFTLNTYN EQQIISDPRL RFELALREAG LHRTLYAKEI LPKISPQKPP RKDMESTVFK I

  • Molecular Weight

    71.0 KDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

CCDC148 (Coevolved Coiled-Coil Domain) is a protein of significant interest due to its involvement in critical cellular processes, including cell division and cytoskeletal organization. Recent studies have highlighted its potential role in mitotic spindle assembly and function, as well as in maintaining genomic stability. Abnormalities in the expression or function of CCDC148 may be linked to various diseases, including cancer, making it a target for therapeutic interventions. The recombinant expression of CCDC148 allows for detailed biochemical characterization and functional assays, contributing to our understanding of its mechanistic roles within the cell. By utilizing advanced techniques in molecular biology, researchers can investigate the structure-function relationships of this protein, providing insights that are essential for unraveling its biological significance. The generation and study of CCDC148 recombinant proteins also pave the way for developing novel biomarkers for disease diagnosis and potential targets for drug development, thus enhancing our overall understanding of cellular dynamics and disease pathogenesis. As research progresses, elucidating the precise roles of CCDC148 in different cellular contexts will be paramount for advancing therapeutic strategies aimed at diseases associated with its dysregulation.

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