Analytical Data
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Gene name
COL4A3
- Application
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Alternative Names
COL4A3；Collagen alpha-3(IV) chain
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q01955
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Expression Region
1428-1670aa
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AA Sequence
LKG KRGDSGSPAT WTTRGFVFTR HSQTTAIPSC PEGTVPLYSG FSFLFVQGNQ RAHGQDLGTL GSCLQRFTTM PFLFCNVNDV CNFASRNDYS YWLSTPALMP MNMAPITGRA LEPYISRCTV CEGPAIAIAV HSQTTDIPPC PHGWISLWKG FSFIMFTSAG SEGTGQALAS PGSCLEEFRA SPFLECHGRG TCNYYSNSYS FWLASLNPER MFRKPIPSTV KAGELEKIIS RCQVCMKKRH
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Molecular Weight
31 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
COL4A3, or collagen type IV alpha 3 chain, is a crucial component of the basal membrane and plays a significant role in maintaining the structural integrity of various tissues, including the kidney, lungs, and blood vessels. Mutations in the COL4A3 gene are associated with several diseases, most notably Alport syndrome, which is characterized by progressive renal failure, hearing loss, and eye abnormalities. The study of recombinant COL4A3 protein has become increasingly important for understanding its functional properties and exploring its potential therapeutic applications. By generating recombinant COL4A3 in various expression systems, researchers can investigate its role in extracellular matrix formation, cell adhesion, and tissue repair processes. Additionally, recombinant COL4A3 serves as a valuable tool for studying pathogenic mechanisms and developing novel treatment strategies for related disorders. As our understanding of COL4A3's molecular mechanics grows, it opens avenues for gene therapies and regenerative medicine, providing hope for patients affected by disorders linked to this essential protein. The ongoing research aims to elucidate not only the fundamental biology of COL4A3 but also to translate these findings into clinical solutions.
