Analytical Data
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Gene name
CASQ1
- Application
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Alternative Names
CASQ1；CASQ；Calsequestrin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31415
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Expression Region
35-396aa
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AA Sequence
QEGLDF PEYDGVDRVI NVNAKNYKNV FKKYEVLALL YHEPPEDDKA SQRQFEMEEL ILELAAQVLE DKGVGFGLVD SEKDAAVAKK LGLTEVDSMY VFKGDEVIEY DGEFSADTIV EFLLDVLEDP VELIEGEREL QAFENIEDEI KLIGYFKSKD SEHYKAFEDA AEEFHPYIPF FATFDSKVAK KLTLKLNEID FYEAFMEEPV TIPDKPNSEE EIVNFVEEHR RSTLRKLKPE SMYETWEDDM DGIHIVAFAE EADPDGFEFL ETLKAVAQDN TENPDLSIIW IDPDDFPLLV PYWEKTFDID LSAPQIGVVN VTDADSVWME MDDEEDLPSA EELEDWLEDV LEGEINTEDD DDDDDD
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CASQ1, or Calsequestrin 1, is a calcium-binding protein predominantly found in the sarcoplasmic reticulum of skeletal muscle cells. Its primary role is to sequester calcium ions, thus regulating intracellular calcium levels which are essential for muscle contraction and relaxation. Research into CASQ1 is gaining momentum due to its crucial involvement in muscle physiology and pathophysiology. Mutations in the CASQ1 gene have been linked to various myopathies, including central core disease and other forms of muscular dystrophy. Understanding the structural and functional dynamics of CASQ1, particularly through recombinant protein studies, can provide valuable insights into its role in muscle function and the mechanisms underlying related diseases. Recombinant CASQ1 proteins enable researchers to explore the protein's biophysical properties, interaction with other sarcoplasmic reticulum proteins, and its regulatory mechanisms in myocyte calcium homeostasis. Furthermore, studies on the recombinant form may facilitate the development of therapeutic strategies for calcium-related muscle disorders. By elucidating how CASQ1 operates at a molecular level, researchers aim to uncover potential targets for intervention in muscular diseases linked to calcium dysregulation. Overall, CASQ1 represents a significant focus of investigation in muscle biology, with implications for both basic research and clinical applications.
