Analytical Data
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Gene name
RDH5
- Application
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Alternative Names
RDH5; HSD17B9; RDH1; SDR9C5; Retinol dehydrogenase 5; EC 1.1.1.209; EC 1.1.1.315; EC 1.1.1.53; 11-cis retinol dehydrogenase; 11-cis RDH; 11-cis RoDH; 9-cis retinol dehydrogenase; 9cRDH; Short chain dehydrogenase/reductase family 9C member 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92781
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Expression Region
24-318 aa
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AA Sequence
LPASNAF VFITGCDSGF GRLLALQLDQ RGFRVLASCL TPSGAEDLQR VASSRLHTTL LDITDPQSVQ QAAKWVEMHV KEAGLFGLVN NAGVAGIIGP TPWLTRDDFQ RVLNVNTMGP IGVTLALLPL LQQARGRVIN ITSVLGRLAA NGGGYCVSKF GLEAFSDSLR RDVAHFGIRV SIVEPGFFRT PVTNLESLEK TLQACWARLP PATQAHYGGA FLTKYLKMQQ RIMNLICDPD LTKVSRCLEH ALTARHPRTR YSPGWDAKLL WLPASYLPAS LVDAVLTWVL PKPAQAVY
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Molecular Weight
34.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RDH5, or Retinol Dehydrogenase 5, is an enzyme primarily involved in the metabolic processes of retinoids, which are vital for vision, growth, and cellular differentiation. Mutations in the RDH5 gene have been linked to a form of retinitis pigmentosa, a degenerative eye disease that leads to vision loss. Understanding RDH5's structure and function through recombinant protein studies is crucial for elucidating the mechanism of retinoid metabolism and the pathogenesis of related retinal diseases. Researchers utilize recombinant DNA technology to produce RDH5 in various expression systems, allowing for the characterization of its enzymatic activity, substrate specificity, and interactions with other proteins. These studies are pivotal for developing potential therapeutic strategies aimed at correcting the enzymatic deficiencies associated with RDH5 mutations, thereby offering hope for treatments of diseases like retinitis pigmentosa. Moreover, exploring the role of RDH5 in cellular retinoid metabolism may lead to broader insights into retinoid-related disorders and contribute to improvements in gene therapy approaches. Overall, research on RDH5 recombinant protein enhances our understanding of retinoid biology and its implications for human health.
