Analytical Data
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Gene name
PARP3
- Application
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Alternative Names
PARP3；ADPRT3；ADPRTL3；Protein mono-ADP-ribosyltransferase PARP3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6F1
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Expression Region
1-533aa
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AA Sequence
MAPKPKPWVQTEGPEKKKGRQAGREEDPFRSTAEALKAIPAEKRIIRVDP TCPLSSNPGTQVYEDYNCTLNQTNIENNNNKFYIIQLLQDSNRFFTCWNH WGRVGEVGQSKINHFTRLEDAKKDFEKKFREKTKNNWAERDHFVSHPGKY TLIEVQAEDEAQEAVVKVDRGPVRTVTKRVQPCSLDPATQKLITNIFSKE MFKNTMALMDLDVKKMPLGKLSKQQIARGFEALEALEEALKGPTDGGQSL EELSSHFYTVIPHNFGHSQPPPINSPELLQAKKDMLLVLADIELAQALQA VSEQEKTVEEVPHPLDRDYQLLKCQLQLLDSGAPEYKVIQTYLEQTGSNH RCPTLQHIWKVNQEGEEDRFQAHSKLGNRKLLWHGTNMAVVAAILTSGLR IMPHSGGRVGKGIYFASENSKSAGYVIGMKCGAHHVGYMFLGEVALGREH HINTDNPSLKSPPPGFDSVIARGHTEPDPTQDTELELDGQQVVVPQGQPV PCPEFSSSTFSQSEYLIYQESQCRLRYLLEVHL
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PARP3 (Poly(ADP-ribose) polymerase 3) is a member of the PARP family, which plays a critical role in DNA damage response and repair mechanisms. Unlike its more extensively studied counterpart PARP1, PARP3 has garnered attention due to its unique functions and potential implications in cancer biology and therapeutic strategies. Research indicates that PARP3 is involved in various cellular processes, including the regulation of DNA strand break repair, modulation of transcriptional activity, and involvement in cellular signaling pathways. Its ability to catalyze the addition of ADP-ribose moieties to target proteins not only contributes to the cellular stress response but also suggests a role in cellular differentiation and apoptosis. As PARP3 interacts with other proteins implicated in DNA repair, understanding its specific mechanisms may unlock new avenues for cancer treatment, especially in PARP inhibitor therapies. The exploration of PARP3’s structural features, enzymatic functions, and interactions within cellular environments is becoming increasingly important, particularly as the intersection between DNA repair pathways and oncogenesis is elucidated. Consequently, investigating PARP3 could lead to novel insights into targeted cancer therapies and enhance our understanding of therapeutic resistance observed in various malignancies.
