Cat: PA2000-2676

Recombinant Methanobacterium nifH1 Protein,His

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Analytical Data

  • 基因名

    nifH1

  • Application

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    nifH1;Nitrogenase iron Protein 1

  • 种属

    Methanobacterium

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P51602

  • 表达区间

    1-275aa

  • 氨基酸序列

    MVRKIAIYGKGGIGKSTTTQNTASAMAHFHNQRVMIHGCDPKADSTRMILGGKMQTTMMDTLREEGEEACMDLDNVMSTGFKDIKCVESGGPEPGVGCAGRGVITAITIMEHMKVYDDNDFVFFDVLGDVVCGGFAMPIRDGKAEEIYIVASGEMMALYAANNLCKGMVKYAEQSGVRLGGIICNSRNVDGEKELLEEFCKRIGTQMIHFVPRDNIVQKAEFNKRTVVDFDAECSQAHEYSELARKIIENDNFVIPDPMTMDELEEMVVSYGLMD

  • 分子量

    37.4 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

The nifH1 gene encodes a key component of nitrogenase, an enzyme critical for biological nitrogen fixation, which allows certain organisms to convert atmospheric nitrogen into ammonia, a form that can be utilized by plants. Research on the nifH1 recombinant protein has gained significant attention due to its potential applications in agriculture and environmental sustainability. By studying this protein, scientists aim to understand the molecular mechanisms underlying nitrogen fixation and the regulation of nitrogenase activity. With the increasing demand for sustainable agricultural practices to support a growing global population, exploring nifH1 and its recombinant forms could lead to the development of biofertilizers or genetically engineered crops that require less chemical nitrogen fertilizers. Additionally, insights gained from nifH1 research can contribute to biotechnological advances in microbial ecology, biodiversity preservation, and the enhancement of soil health. The ability to produce and characterize recombinant nifH1 proteins in various expression systems opens up opportunities for structural studies, functional assays, and the exploration of its interaction with other components of the nitrogen-fixing machinery. Overall, the investigation of the nifH1 recombinant protein stands at the intersection of applied microbiology, biochemistry, and agricultural science, with the potential to address both food security and environmental challenges.

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IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
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