Analytical Data
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Gene name
C9orf32
- Application
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Alternative Names
AD 003; Alpha N-terminal Protein methyltransferase 1A; C9orf32; Chromosome 9 open reading frame 32; Methyltransferase like 11A; Methyltransferase-like Protein 11A; Mettl11a; N-terminal RCC1 methyltransferase; NRMT
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BV86
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Expression Region
1-223aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTSEV IEDEKQFYSK AKTYWKQIPP TVDGMLGGYG HISSIDINSS RKFLQRFLRE GPNKTGTSCA LDCGAGIGRI TKRLLLPLFR EVDMVDITED FLVQAKTYLG EEGKRVRNYF CCGLQDFTPE PDSYDVIWIQ WVIGHLTDQH LAEFLRRCKG SLRPNGIIVI KDNMAQEGVI LDDVDSSVCR DLDVVRRIIC SAGLSLLAEE RQENLPDEIY HVYSFALR
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Molecular Weight
28 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
C9orf32, or Chromosome 9 Open Reading Frame 32, is a gene that has garnered significant attention in the field of neurodegenerative disease research, particularly in relation to amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The emergence of hexanucleotide repeat expansions (GGGGCC) within the intron of the C9orf32 gene has been identified as a major genetic cause of these disorders, affecting a substantial proportion of familial cases. The resulting protein product, although still not fully understood, is believed to play a crucial role in cellular processes such as RNA metabolism and stress response. Research studies have increasingly focused on the biophysical and biochemical properties of recombinant C9orf32 protein to elucidate its pathogenic mechanisms and interactions within the neuronal environment. Understanding the structure and function of the C9orf32 protein can provide insights into the molecular pathways leading to neurodegeneration, potentially paving the way for the development of targeted therapeutic strategies. Furthermore, examining the effects of C9orf32 protein disruptions can help delineate the interplay between genetic mutations and environmental factors in the onset of neurodegenerative diseases. Overall, investigations into C9orf32 and its recombinant protein are essential for advancing our knowledge of ALS and FTD, ultimately contributing to improved diagnosis and treatment options for affected individuals.
