Analytical Data
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Gene name
CA5A
- Application
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Alternative Names
CA5A；Collagen alpha-1(V) chain
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35218
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Expression Region
39-305aa
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AA Sequence
CAWQTSNNTLHPLWTVPVSVPGGTRQSPINIQWRDSVYDPQLKPLRVSYEAASCLYIWNTGYLFQVEFDDATEASGISGGPLENHYRLKQFHFHWGAVNEGGSEHTVDGHAYPAELHLVHWNSVKYQNYKEAVVGENGLAVIGVFLKLGAHHQTLQRLVDILPEIKHKDARAAMRPFDPSTLLPTCWDYWTYAGSLTTPPLTESVTWIIQKEPVEVAPSQLSAFRTLLFSALGEEEKMMVNNYRPLQPLMNRKVWASFQATNEGTRS
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Molecular Weight
30.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CA5A, or Carbonic Anhydrase 5A, is an important enzyme belonging to the carbonic anhydrase family, which plays a crucial role in various physiological processes, including ion transport and pH regulation. This particular isoform is primarily expressed in the mitochondria and is involved in metabolic pathways that influence cellular respiration and energy production. The study of CA5A is significant as it has been linked to several pathological conditions, such as metabolic disorders, cancer, and mitochondrial diseases. Research has indicated that CA5A may participate in the regulation of bicarbonate levels and can affect the activity of other enzymes in metabolic pathways. Investigating the recombinant form of CA5A allows scientists to better understand its structure-function relationships and enzymatic mechanisms. By producing recombinant CA5A, researchers can explore its enzymatic properties, stability, and interactions with inhibitors, which may lead to potential therapeutic applications. Furthermore, studying CA5A's role in cellular metabolism may provide insights into its contribution to disease states, emphasizing the need for continued research in this area to develop targeted strategies for treatment and prevention. Overall, the exploration of CA5A through recombinant protein research represents a promising avenue for enhancing our understanding of its biological significance and therapeutic potential.
