Analytical Data
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Gene name
STE13
- Application
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Alternative Names
STE13；dhh1；Putative ATP-dependent RNA helicase ste13
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33894
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Expression Region
1-119aa
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AA Sequence
MSASTHSHKRKNSHLFPQRKSSNSSMDKPFFPNNDSVANTDPQSNENGHTINEIRPTEATIDVTDVPQTPFLQEQYSMRPRRESFQFNDIENQHHTHSFFSVNKFNRRWGEWSLPEKRS
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Molecular Weight
18.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the STE13 recombinant protein is rooted in the interest of understanding its role within the yeast Saccharomyces cerevisiae, particularly in the context of mating and cell signaling pathways. STE13 encodes a protein involved in the processing of pheromones, which are crucial for yeast mating and communication. Previous research has shown that mutations or deficiencies in STE13 can lead to impaired mating responses, highlighting its significance in the regulation of mating-type switching and pheromone perception. Moreover, the STE13 protein is part of a larger family of proteins involved in the proteolytic cleavage of precursor peptides, impacting the overall signaling mechanisms within the yeast cells. The recombinant expression of STE13 allows for the detailed study of its biochemical properties, functional activity, and interactions with other signaling molecules. This research not only deepens our understanding of yeast biology but also has broader implications in the field of cell signaling, with potential applications in biotechnology and medical research where similar pathways are relevant. By elucidating the mechanisms by which STE13 functions, scientists aim to uncover novel insights into cellular communication processes that may extend beyond fungal systems, providing a basis for future studies in eukaryotic cell biology and potential therapeutic targets.
