Analytical Data
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Gene name
RASGEF1A
- Application
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Alternative Names
CG4853 gene product ; Ras-GEF domain-containing family member 1A; RasGEF domain family. member 1A; rasgef1a; RGF1A_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N9B8
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Expression Region
1-481 aa
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AA Sequence
MPQTSVVFSS ILGPSCSGQV QPGMGERGGG AGGGSGDLIF QDGHLISGSL EALMEHLVPT VDYYPDRTYI FTFLLSSRVF MPPHDLLARV GQICVEQKQQ LEAGPEKAKL KSFSAKIVQL LKEWTEAFPY DFQDEKAMAE LKAITHRVTQ CDEENGTVKK AIAQMTQSLL LSLAARSQLQ ELREKLRPPA VDKGPILKTK PPAAQKDILG VCCDPLVLAQ QLTHIELDRV SSIYPEDLMQ IVSHMDSLDN HRCRGDLTKT YSLEAYDNWF NCLSMLVATE VCRVVKKKHR TRMLEFFIDV ARECFNIGNF NSMMAIISGM NLSPVARLKK TWSKVKTAKF DVLEHHMDPS SNFCNYRTAL QGATQRSQMA NSSREKIVIP VFNLFVKDIY FLHKIHTNHL PNGHINFKKF WEISRQIHEF MTWTQVECPF EKDKKIQSYL LTAPIYSEEA LFVASFESEG PENHMEKDSW KTLRTTLLNR A
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Molecular Weight
54.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RASGEF1A, a member of the RAS guanine nucleotide exchange factor family, plays a crucial role in cellular signaling pathways by facilitating the activation of RAS proteins, which are pivotal in regulating cell growth, differentiation, and survival. Recent studies have highlighted the significance of RASGEF1A in various physiological processes and its potential implications in cancer biology. Aberrant activation of RAS signaling is a common feature in numerous malignancies, making the study of RASGEF1A particularly relevant for understanding tumorigenesis and identifying therapeutic targets. Researchers are increasingly focused on characterizing RASGEF1A through recombinant protein studies, aiming to elucidate its molecular mechanisms and interactions with RAS and other signaling molecules. The heterogeneity of RAS protein family members and their distinct but overlapping functions add complexity to the understanding of RASGEF1A’s role in cellular contexts. Advanced techniques, such as mutagenesis and structural biology, are being employed to investigate the functional domains of RASGEF1A, assess its activity as a GEF, and explore its potential as a drug target. This research not only provides insights into RASGEF1A's biological function but also contributes to the broader landscape of targeted therapies in oncology, paving the way for innovative treatment strategies that leverage our understanding of RAS signaling pathways. As the field evolves, the study of RASGEF1A and its recombination continues to hold promise for both basic research and clinical applications, underscoring the importance of detailed protein analysis in the quest for novel cancer therapies.
